Abstract

The present study, the protective effect of Olea europaea L. leaf extract containing high oleuropein on oxidative damage in ischemic and non-ischemic parts of rat liver was investigated. The Spraque-dawley male rats were separated into five groups. The physiological water was given to Group I and Group II. Group III, IV, and V animals received orally 150, 200 and 400 mg kg^–1/day olive leaf extract for 15 days, respectively. Then, except for Group I rats, partial (2/3) liver Ischemia (45 min)/Reperfusion (60 min) (I/R) was applied in the other groups. In addition to histopathological and cytological examination, serum Alanine transaminase (ALT) and Aspartate transaminase (AST) levels in liver tissues as well as activities of the Superoxide dismutase (SOD), Catalase (CAT) and Glutathione peroxidase (GPx) enzymes were investigated. As a result of the statistical evaluations of all parameters, the oxidative damage in ischemic and non-ischemic parts of the liver was same in all experimental groups. 200 mg kg^–1/day Olea europaea L. leaf extract was found to have more protective effect against I/R induced oxidative damage when compared to other doses.

中文翻译：

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用油橄榄叶提取物预防部分肝脏缺血/再灌注后组织损伤

摘要

本研究考察了含有高橄榄苦苷的油橄榄叶提取物对大鼠肝脏缺血和非缺血部位氧化损伤的保护作用。将斯普拉克-道利雄性大鼠分成五组。第I组和第II组给予生理水。III、IV 和 V 组动物口服 150、200 和 400 mg kg ^–1/天橄榄叶提取物，分别为 15 天。然后，除I组大鼠外，在其他组中应用部分（2/3）肝缺血（45分钟）/再灌注（60分钟）（I / R）。除了组织病理学和细胞学检查外，还研究了肝组织中血清丙氨酸转氨酶 (ALT) 和天冬氨酸转氨酶 (AST) 水平以及超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT) 和谷胱甘肽过氧化物酶 (GPx) 酶的活性。作为所有参数的统计评估结果，肝脏缺血和非缺血部位的氧化损伤在所有实验组中是相同的。与其他剂量相比，发现200 mg kg ^–1 / 天的油橄榄叶提取物对 I/R 诱导的氧化损伤具有更强的保护作用。