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Prevention of Tissue Injury with Olea europaea L. Leaf Extract after Partial Liver Ischemia/Reperfusion

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Abstract

The present study, the protective effect of Olea europaea L. leaf extract containing high oleuropein on oxidative damage in ischemic and non-ischemic parts of rat liver was investigated. The Spraque-dawley male rats were separated into five groups. The physiological water was given to Group I and Group II. Group III, IV, and V animals received orally 150, 200 and 400 mg kg–1/day olive leaf extract for 15 days, respectively. Then, except for Group I rats, partial (2/3) liver Ischemia (45 min)/Reperfusion (60 min) (I/R) was applied in the other groups. In addition to histopathological and cytological examination, serum Alanine transaminase (ALT) and Aspartate transaminase (AST) levels in liver tissues as well as activities of the Superoxide dismutase (SOD), Catalase (CAT) and Glutathione peroxidase (GPx) enzymes were investigated. As a result of the statistical evaluations of all parameters, the oxidative damage in ischemic and non-ischemic parts of the liver was same in all experimental groups. 200 mg kg–1/day Olea europaea L. leaf extract was found to have more protective effect against I/R induced oxidative damage when compared to other doses.

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Funding

This study was supported by Eskisehir Osmangazi University Research Fund by project no. 201319A204.

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  1. Department of Biology, Faculty of Science and Letters, Eskisehir Osmangazi University, TR-26040, Eskisehir, Turkey

    Mustafa Uyanoğlu

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  1. Mustafa Uyanoğlu
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Correspondence to Mustafa Uyanoğlu.

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The author declares no conflict of interest. This article does not contain any studies involving animals or human participants performed by the author.

The experimental protocols were approved by the Institutional Ethical Committee for Animal Care and Use at Eskisehir Osmangazi University, Eskisehir, Turkey (protocol number: 2013/336).

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Mustafa Uyanoğlu Prevention of Tissue Injury with Olea europaea L. Leaf Extract after Partial Liver Ischemia/Reperfusion. Biol Bull Russ Acad Sci 48, 536–545 (2021). https://doi.org/10.1134/S1062359021050150

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