The evolution of antimicrobial-resistant pathogens is a global health and development threat. Nanomedicine is rapidly becoming the main driving force behind ongoing changes in antimicrobial studies. Among nanoparticles, silver (AgNPs) have attracted attention due to their versatile properties. The study aimed to investigate the effects of AgNPs and L-carnitine (LC) on mixed Candida albicans and Staphylococcus aureus in the mice vaginitis model. Study of antimicrobial activity of AgNPs evaluated by Minimum Inhibitory Concentration (MIC) and Minimum Biocidal Concentration (MBC) assays. AgNPs inhibited biofilm formation of microbial strains, which was tested by using crystal violet staining. In the current study, we evaluated the effects of AgNPs and LC in NMRI mice infected intravaginally with C. albicans/ S. aureus for two weeks. The proportion of mice in each stage of the estrous cycle (proestrus, estrus, metestrus, and diestrus) was examined. Histological properties were assessed by hematoxylin/ eosin (H&E) staining of formalin-fixed, paraffin-embedded vaginal tissue sections. Based on the results, MICs of AgNPs against S. aureus, C. albicans, and their combination were 252.3, 124.8, and 501.8 ppm, and their minimum biofilm inhibitory concentration (MBIC) was 500, 250, and 1000 ppm, respectively. The estrous cycle in the treated group was similar to the control. Vaginal histology and cytology showed that LC can improve tissue damages caused by vaginitis and AgNPs. This study demonstrates the promising use of AgNPs as antimicrobial agents and the combination of AgNPs/ LC could be a great future alternative in the control of vaginitis.

抗微生物病原体的进化是全球健康和发展的威胁。纳米医学正迅速成为抗菌研究不断变化的主要推动力。在纳米粒子中，银（AgNPs）因其多功能特性而备受关注。该研究旨在研究 AgNPs 和左旋肉碱 (LC) 对混合白色念珠菌和金黄色葡萄球菌的影响在小鼠阴道炎模型中。通过最低抑菌浓度 (MIC) 和最低杀生物浓度 (MBC) 测定评估 AgNP 的抗菌活性的研究。AgNPs 抑制了微生物菌株的生物膜形成，这通过使用结晶紫染色进行了测试。在当前的研究中，我们评估了 AgNPs 和 LC 对阴道内感染白色念珠菌/金黄色葡萄球菌两周的NMRI 小鼠的影响。检查了发情周期每个阶段（发情前期、发情期、发情期和发情期）中小鼠的比例。通过福尔马林固定、石蜡包埋的阴道组织切片的苏木精/伊红 (H&E) 染色评估组织学特性。根据结果​​，AgNPs 对金黄色葡萄球菌、白色念珠菌的MIC，它们的组合分别为 252.3、124.8 和 501.8 ppm，它们的最小生物膜抑制浓度 (MBIC) 分别为 500、250 和 1000 ppm。治疗组的动情周期与对照组相似。阴道组织学和细胞学表明，LC可以改善阴道炎和AgNPs引起的组织损伤。这项研究表明，AgNPs 作为抗菌剂很有前景，AgNPs/LC 的组合可能是未来控制阴道炎的一个很好的替代方案。