Green Synthesis, Characterization and Antibacterial Activity of SiO2–ZnO Nanocomposite by Dictyota bartayresiana Extract and Its Cytotoxic Effect on HT29 Cell Line,Journal of Cluster Science

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Green Synthesis, Characterization and Antibacterial Activity of SiO2–ZnO Nanocomposite by Dictyota bartayresiana Extract and Its Cytotoxic Effect on HT29 Cell Line
Journal of Cluster Science ( IF 2.7 ) Pub Date : 2021-09-19 , DOI: 10.1007/s10876-021-02170-w
D. Swarna Bharathi ₁ , A. Boopathyraja ₁ , Suganthi Nachimuthu ₂ , Karthik Kannan ₃

Affiliation

This study elucidates the biosynthesis of two various metal nanoparticles using brown seaweed algae from the southeast coast region of Tamil Nadu. The synthesized SiO₂–ZnO nanocomposite was analyzed using UV–Vis, SEM, FT-IR and XRD analysis. The biomedical studies of synthesized SiO₂–ZnO nanocomposite on antioxidant, antibacterial and anticancer activity were evaluated. Antioxidant screening assays were performed by using nitric oxide, hydrogen peroxide and ABTS assay. MTT assay and well-cut drug diffusion assay were performed for anticancer and antibacterial studies. The bio-synthesized SiO₂–ZnO nanocomposites have acted as a promising potential drug against HT29 cancer cell line of colon cancer and explore the best range of zone inhibition upon gram-negative and gram-positive bacterial strains. The results provide the facts that the seaweed mediated synthesis of SiO₂–ZnO nanocomposite might be the potential source to treat adenorectal colon cancer cell and exhibits significant effect on Escherichia coli, Vibrio cholera, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus. This study confirms that seaweed extract mediated synthesis of SiO₂–ZnO nanocomposites significantly inhibits bacterial colonies especially urinary tract infection pathogens and shows excellent antioxidant activity.

Graphic Abstract

Schematic representation of synthesized SiO₂–ZnO nanocomposites and their therapeutic evaluation.

中文翻译：

巴旦木提取物的 SiO2-ZnO 纳米复合材料的绿色合成、表征和抗菌活性及其对 HT29 细胞系的细胞毒作用

这项研究阐明了使用来自泰米尔纳德邦东南沿海地区的棕色海藻生物合成两种不同的金属纳米粒子。使用UV-Vis、SEM、FT-IR和XRD分析对合成的SiO ₂ -ZnO纳米复合材料进行分析。评价了合成的 SiO ₂ -ZnO 纳米复合材料的抗氧化、抗菌和抗癌活性的生物医学研究。通过使用一氧化氮、过氧化氢和ABTS测定进行抗氧化剂筛选测定。MTT 测定和良好切割的药物扩散测定用于抗癌和抗菌研究。生物合成 SiO ₂– ZnO 纳米复合材料已作为一种有前景的潜在药物来对抗结肠癌的 HT29 癌细胞系，并探索对革兰氏阴性和革兰氏阳性细菌菌株的最佳区域抑制范围。结果表明，海藻介导的SiO ₂ -ZnO纳米复合材料的合成可能是治疗腺直肠结肠癌细胞的潜在来源，对大肠杆菌、霍乱弧菌、铜绿假单胞菌、枯草芽孢杆菌和金黄色葡萄球菌有显着影响。该研究证实，海藻提取物介导了 SiO _{2 的}合成–ZnO 纳米复合材料显着抑制细菌菌落，尤其是尿路感染病原体，并显示出优异的抗氧化活性。