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Overexpression of seagrass DnaJ gene ZjDjB1 enhances the thermotolerance of transgenic arabidopsis thaliana
Physiology and Molecular Biology of Plants ( IF 3.4 ) Pub Date : 2021-09-18 , DOI: 10.1007/s12298-021-01063-6
Siting Chen 1 , Guanglong Qiu 1
Affiliation  

Seagrass meadows are one of the most important marine resources that grow along the coast. They provide habitat and a food source for animals. They also protect the coast, fix sediment and purify seawater. In the current period of global climate change, anomalies in coastal water temperatures are increasing. A sudden increase in water temperature owing to a heat wave can have a profound effect on seagrass. Zostera japonica is a type of intertidal seagrasses, which is exposed to the air at low tide. High temperatures in the summer often lead to a decline in seagrass meadows. DnaJ proteins, also known as J proteins, are a family of conserved chaperone proteins. They are designated as J proteins because they contain a highly conserved J domain. They function as chaperones of heat shock proteins in organisms. In this study, the role of DnaJ protein (ZjDjB1) of Z. japonica under heat stress was studied. ZjDjB1 was localized to the cytoplasm and nucleus. The overexpression of ZjDjB1 in Arabidopsis thaliana results in an increase in thermotolerance and a decrease in the accumulation of reactive oxygen species and also a reduction in membrane damage. ZjDjB1 may achieve this goal by maintaining a low activity of proteolytic enzymes.



中文翻译:

海草DnaJ基因ZjDjB1过表达增强转基因拟南芥耐热性

海草草甸是沿海岸生长的最重要的海洋资源之一。它们为动物提供栖息地和食物来源。它们还保护海岸、固定沉积物和净化海水。在当前全球气候变化时期,沿海水温异常正在增加。热浪引起的水温突然升高会对海草产生深远的影响。粳稻是一种潮间带海草,在退潮时暴露在空气中。夏季高温常导致海草草地减少。DnaJ 蛋白,也称为 J 蛋白,是一个保守的伴侣蛋白家族。它们被称为 J 蛋白,因为它们含有高度保守的 J 结构域。它们在生物体中充当热激蛋白的伴侣。本研究研究了粳稻DnaJ蛋白(ZjDjB1)在热胁迫下的作用。ZjDjB1 定位于细胞质和细胞核。ZjDjB1拟南芥中的过表达导致耐热性的增加和活性氧的积累减少以及膜损伤的减少。ZjDjB1可以通过保持蛋白水解酶的低活性来实现这一目标。

更新日期:2021-09-19
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