Abstract
Seagrass meadows are one of the most important marine resources that grow along the coast. They provide habitat and a food source for animals. They also protect the coast, fix sediment and purify seawater. In the current period of global climate change, anomalies in coastal water temperatures are increasing. A sudden increase in water temperature owing to a heat wave can have a profound effect on seagrass. Zostera japonica is a type of intertidal seagrasses, which is exposed to the air at low tide. High temperatures in the summer often lead to a decline in seagrass meadows. DnaJ proteins, also known as J proteins, are a family of conserved chaperone proteins. They are designated as J proteins because they contain a highly conserved J domain. They function as chaperones of heat shock proteins in organisms. In this study, the role of DnaJ protein (ZjDjB1) of Z. japonica under heat stress was studied. ZjDjB1 was localized to the cytoplasm and nucleus. The overexpression of ZjDjB1 in Arabidopsis thaliana results in an increase in thermotolerance and a decrease in the accumulation of reactive oxygen species and also a reduction in membrane damage. ZjDjB1 may achieve this goal by maintaining a low activity of proteolytic enzymes.
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Data availability
All data generated or analyzed during this study are included in this published article. The nucleotide and deduced amino acid sequence data of ZjDjB1 were registered in the GenBank (No. MN395290).
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Funding
This study was funded by the Natural Science Foundation of Guangxi Province (2020GXNSFAA297067; 2018GXNSFBA281062), the Research Fund Program of Guangxi Key Lab of Mangrove Conservation and Utilization (Grant No. GKLMC-20A04, GKLMC-20A01); the Distinguished Employment Offered Unit of Guangxi for Conservation and Ecological Monitoring of Mangroves and Seagrasses, the National Science & Technology Basic Work Program (2015FY110600) and the National Key Research and Development Program of China (2017YFC0506100). These funding bodies had no role in the design of the study, collection, analysis, and interpretation of data, or in the writing of the manuscript.
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SC designed the experiment and carry out the experiment. GQ conducted field sampling and identification. SC and GQ wrote the manuscript. All the authors reviewed the manuscript.
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Chen, S., Qiu, G. Overexpression of seagrass DnaJ gene ZjDjB1 enhances the thermotolerance of transgenic arabidopsis thaliana. Physiol Mol Biol Plants 27, 2043–2055 (2021). https://doi.org/10.1007/s12298-021-01063-6
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DOI: https://doi.org/10.1007/s12298-021-01063-6