The entomopathogenic fungus Beauveria bassiana is used worldwide for biological control of insects. Seven dsRNA segments were detected in a single B. bassiana strain, RCEF1446. High-throughput sequencing indicated the presence of three mycoviruses in RCEF1446. Two were identified as the known mycoviruses Beauveria bassiana victorivirus 1 and Beauveria bassiana polymycovirus 1, and the novel mycovirus was designated as "Beauveria bassiana bipartite mycovirus 1" (BbBV1). The complete sequence of the BbBV1 is described here. The mycovirus contains two dsRNA segments. The RNA 1 (dsRNA 4) of BbBV1 is 2,026 bp in length, encoding a RNA-dependent RNA polymerase (RdRp) (68.54 kDa), while the RNA 2 (dsRNA 6) is 1,810 bp in length, encoding a hypothetical protein (35.55 kDa) with unknown function. Moreover, the amino acid sequence of RdRp showed the highest sequence identity of 62.31% to Botryosphaeria dothidea bipartite mycovirus 1. Phylogenetic analysis based on RdRp sequences revealed that BbBV1 represents a distinct lineage of unassigned dsRNA mycoviruses infecting fungi.

昆虫病原真菌白僵菌在世界范围内用于昆虫的生物防治。在单个B. bassiana中检测到七个 dsRNA 片段菌株，RCEF1446。高通量测序表明 RCEF1446 中存在三种真菌病毒。其中两种被鉴定为已知的霉菌病毒 Beauveria bassiana victorivirus 1 和 Beauveria bassiana polymycovirus 1，新型霉菌病毒被命名为“Beauveria bassiana bipartite mycovirus 1”（BbBV1）。此处描述了 BbBV1 的完整序列。真菌病毒包含两个 dsRNA 片段。BbBV1 的 RNA 1 (dsRNA 4) 长 2,026 bp，编码 RNA 依赖性 RNA 聚合酶 (RdRp) (68.54 kDa)，而 RNA 2 (dsRNA 6) 长 1,810 bp，编码假设蛋白质 (35.55 kDa) 功能未知。此外，RdRp 的氨基酸序列与 Botryosphaeria dothidea 二分枝霉菌病毒 1 的序列同一性最高，为 62.31%。