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Molecular characterization of two dsRNAs that could correspond to the genome of a new mycovirus that infects the entomopathogenic fungus Beauveria bassiana

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Abstract

The entomopathogenic fungus Beauveria bassiana is used worldwide for biological control of insects. Seven dsRNA segments were detected in a single B. bassiana strain, RCEF1446. High-throughput sequencing indicated the presence of three mycoviruses in RCEF1446. Two were identified as the known mycoviruses Beauveria bassiana victorivirus 1 and Beauveria bassiana polymycovirus 1, and the novel mycovirus was designated as "Beauveria bassiana bipartite mycovirus 1" (BbBV1). The complete sequence of the BbBV1 is described here. The mycovirus contains two dsRNA segments. The RNA 1 (dsRNA 4) of BbBV1 is 2,026 bp in length, encoding a RNA-dependent RNA polymerase (RdRp) (68.54 kDa), while the RNA 2 (dsRNA 6) is 1,810 bp in length, encoding a hypothetical protein (35.55 kDa) with unknown function. Moreover, the amino acid sequence of RdRp showed the highest sequence identity of 62.31% to Botryosphaeria dothidea bipartite mycovirus 1. Phylogenetic analysis based on RdRp sequences revealed that BbBV1 represents a distinct lineage of unassigned dsRNA mycoviruses infecting fungi.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant Nos. 31772226, 31471821 and 30770008).

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Author notes

  1. Najie Shi and Fei Hu contributed equally to this study and share first authorship.

Authors and Affiliations

  1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China

    Najie Shi, Ping Wang, Yuxiang Zhang, Qiuyan Zhu & Bo Huang

  2. Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, 230031, China

    Fei Hu

  3. School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China

    Guogen Yang

Authors
  1. Najie Shi
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  2. Fei Hu
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  3. Ping Wang
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  4. Yuxiang Zhang
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  5. Qiuyan Zhu
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  6. Guogen Yang
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  7. Bo Huang
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Correspondence to Bo Huang.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Robert H.A. Coutts.

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Shi, N., Hu, F., Wang, P. et al. Molecular characterization of two dsRNAs that could correspond to the genome of a new mycovirus that infects the entomopathogenic fungus Beauveria bassiana. Arch Virol 166, 3233–3237 (2021). https://doi.org/10.1007/s00705-021-05239-z

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  • DOI: https://doi.org/10.1007/s00705-021-05239-z

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