Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2021-09-18 , DOI: 10.1007/s00216-021-03660-6 Wenxiang Xiao 1 , Jing Liu 1 , Yinan Xiong 1 , Yaoxin Li 1 , Hua Li 1
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Serum bilirubin is an important indicator to assess liver function and diagnose various types of liver diseases. The level of serum bilirubin is also negatively correlated with the risk of cardiovascular disease and cancer. We had fabricated a fluorescent film sensor aiming at free bilirubin detection at the nanomolar level. Gold nanoclusters capped by human serum albumin (HSA–AuNCs) were utilized as a fluorescent platform for bilirubin biorecognition. HSA–AuNCs were functionalized with glucuronic acid to increase the binding sites for bilirubin. An ultrathin film of glucuronic acid–functionalized gold nanoclusters was obtained by the Langmuir-Blodgett (LB) technique. When exposed to bilirubin, the interaction between free bilirubin and the functionalized AuNCs resulted in fluorescent quenching of the film. Good linearity could be achieved for the quenching efficiency versus the logarithm of free bilirubin concentration over a concentration range of 1.00 nM~5.00 μM. The limit of detection (LOD) was calculated to be (2.70 ± 0.14) × 10−1 nM (S/N = 3). The film sensor presents a good anti-interference capability towards common substances coexisting with bilirubin in serum. Satisfactory results achieved in the tests of real serum samples indicate that the LB film sensor can be used for bilirubin determination in nanomolar concentration.
Graphical abstract
中文翻译：
使用葡糖醛酸功能化金纳米团簇的 Langmuir-Blodgett 薄膜对纳摩尔水平的游离胆红素进行荧光传感
血清胆红素是评估肝功能和诊断各类肝病的重要指标。血清胆红素水平也与心血管疾病和癌症的风险呈负相关。我们制造了一种荧光薄膜传感器，旨在检测纳摩尔水平的游离胆红素。由人血清白蛋白（HSA-AuNCs）覆盖的金纳米簇被用作胆红素生物识别的荧光平台。HSA-AuNCs 用葡萄糖醛酸功能化以增加胆红素的结合位点。通过 Langmuir-Blodgett (LB) 技术获得了葡糖醛酸功能化金纳米团簇的超薄膜。当暴露于胆红素时，游离胆红素和功能化的 AuNC 之间的相互作用导致薄膜的荧光猝灭。在 1.00 nM~5.00 μM 的浓度范围内，淬灭效率与游离胆红素浓度的对数关系可以实现良好的线性关系。检测限 (LOD) 计算为 (2.70 ± 0.14) × 10-1 nM ( S / N = 3)。该薄膜传感器对血清中与胆红素共存的常见物质具有良好的抗干扰能力。在真实血清样品的测试中取得了令人满意的结果，表明 LB 薄膜传感器可用于纳摩尔浓度的胆红素测定。
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