Arsenic is considered a worldwide pollutant that can be present in drinking water. Arsenic exposure is associated with various diseases, including cancer. Antioxidants as selenite and α-tocopherol-succinate have been shown to modulate arsenic toxic effects. Since changes in STAT3 and PSMD10 gene expression have been associated with carcinogenesis, the aim of this study was to evaluate the effect of arsenic exposure and co-treatments with selenite or α-tocopherol-succinate on the expression of these genes, in the livers of chronically exposed Syrian golden hamsters. Animals were divided into six groups: (i) control, (ii) chronically treated with 100 ppm arsenic, (iii) treated with 6 ppm α-tocopherol-succinate (α-TOS), (iv) treated with 8.5 ppm selenite, (v) treated with arsenic + α-TOS, and (vi) treated with arsenic + selenite. Urine samples and livers were collected after 20 weeks of continuous exposure. The urine samples were analyzed for arsenic species by atomic absorption spectrophotometry, and real-time RT-qPCR analysis was performed for gene expression evaluation. A reduction in STAT3 expression was observed in the selenite-treated group. No differences in PSMD10 expression were found among groups. Histopathological analysis revealed hepatic lymphocytosis in selenite-treated animals. As a conclusion, long-term exposure to arsenic does not significantly alter the expression of STAT3 and PSMD10 oncogenes in the livers of hamsters; however, selenite down-regulates STAT3 expression and provokes lymphocytosis.

中文翻译：

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亚硒酸盐下调长期暴露的叙利亚金仓鼠肝脏中的 STAT3 表达并引起淋巴细胞增多

砷被认为是一种全球性的污染物，可存在于饮用水中。砷暴露与多种疾病有关，包括癌症。亚硒酸盐和α-生育酚-琥珀酸盐等抗氧化剂已被证明可以调节砷的毒性作用。由于STAT3和PSMD10 的变化基因表达与致癌作用有关，本研究的目的是评估砷暴露以及与亚硒酸盐或α-生育酚-琥珀酸盐共同治疗对长期暴露的叙利亚金仓鼠肝脏中这些基因表达的影响。动物分为六组：（i）对照，（ii）用 100 ppm 砷长期治疗，（iii）用 6 ppm α-生育酚琥珀酸酯（α-TOS）治疗，（iv）用 8.5 ppm 亚硒酸盐治疗，（ v) 用砷 + α-TOS 处理，和 (vi) 用砷 + 亚硒酸盐处理。在连续接触 20 周后收集尿液样本和肝脏。通过原子吸收分光光度法分析尿液样本中的砷种类，并进行实时 RT-qPCR 分析以评估基因表达。STAT3减少在亚硒酸盐处理组中观察到表达。在无差异PSMD10表达组中被发现。组织病理学分析显示亚硒酸盐处理的动物肝脏淋巴细胞增多。总之，长期接触砷不会显着改变仓鼠肝脏中STAT3和PSMD10致癌基因的表达；然而，亚硒酸盐会下调STAT3表达并引起淋巴细胞增多。