Corallococcus sp. strain EGB is a Gram-negative myxobacteria isolated from saline soil, and has considerable potential for the biocontrol of phytopathogenic fungi. However, the detailed mechanisms related to development and predatory behavior are unclear. To obtain a comprehensive overview of genetic features, the genome of strain EGB was sequenced, annotated, and compared with 10 other Corallococcus species. The strain EGB genome was assembled as a single circular chromosome of 9.4 Mb with 7916 coding genes. Phylogenomics analysis showed that strain EGB was most closely related to Corallococcus interemptor AB047A, and it was inferred to be a novel species within the Corallococcus genus. Comparative genomic analysis revealed that the pan-genome of Corallococcus genus was large and open. Only a small proportion of genes were specific to strain EGB, and most of them were annotated as hypothetical proteins. Subsequent analyses showed that strain EGB produced abundant extracellular enzymes such as chitinases and β-(1,3)-glucanases, and proteases to degrade the cell-wall components of phytopathogenic fungi. In addition, 35 biosynthetic gene clusters potentially coding for antimicrobial compounds were identified in the strain EGB, and the majority of them were present in the dispensable pan-genome with unexplored metabolites. Other genes related to secretion and regulation were also explored for strain EGB. This study opens new perspectives in the greater understanding of the predatory behavior of strain EGB, and facilitates a potential application in the biocontrol of fungal plant diseases in the future.

中文翻译：

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Corallococcus sp的全基因组测序。菌株 EGB 揭示了将分类学和捕食行为联系起来的遗传决定因素

珊瑚球菌属 EGB菌株是从盐渍土中分离出来的革兰氏阴性粘杆菌，在植物病原真菌的生物防治方面具有相当大的潜力。然而，与发育和掠夺行为相关的详细机制尚不清楚。为了全面了解遗传特征，对 EGB 菌株的基因组进行了测序、注释，并与其他 10种珊瑚球菌进行了比较。菌株 EGB 基因组被组装成一个 9.4 Mb 的环状染色体，具有 7916 个编码基因。系统基因组学分析表明，菌株EGB与中间珊瑚球菌AB047A的亲缘关系最为密切，推测其为珊瑚球菌属中的一个新种。属。比较基因组分析揭示了珊瑚球菌的泛基因组属大而开放。只有一小部分基因是 EGB 菌株特有的，其中大部分被注释为假设的蛋白质。随后的分析表明，EGB菌株产生丰富的细胞外酶，如几丁质酶和β-(1,3)-葡聚糖酶，以及降解植物病原真菌细胞壁成分的蛋白酶。此外，在菌株 EGB 中鉴定了 35 个可能编码抗菌化合物的生物合成基因簇，其中大多数存在于具有未开发代谢物的可有可无的泛基因组中。其他与分泌和调节相关的基因也被用于菌株 EGB。该研究为更好地理解 EGB 菌株的捕食行为开辟了新的视角，并促进了未来在真菌植物病害生物防治中的潜在应用。