COVID-19 caused by SARS-CoV-2 caused a worldwide crisis, highlighting the importance of preventive measures in infectious diseases control. SARS-CoV-2 can remain infectious on surfaces for up to several weeks; therefore, proper disinfection is required to mitigate the risk of indirect virus spreading. Gaseous ozone treatment has received particular attention as an easily accessible disinfection tool. In this study, we evaluated the virucidal effectiveness of gaseous ozone treatment (>7.3 ppm, 2 h) on MHV-contaminated stainless-steel surface and PBS-suspended virus under field conditions at ambient (21.8%) and high (49.8–54.2%) relative humidity. Surficial virus was soiled with 0.3 g/l of BSA. Parallelly, a half-hour vaporization with 7.3% hydrogen peroxide was performed on contaminated carriers. The obtained results showed that gaseous ozone, whilst quite effective against suspended virus, was insufficient in sanitizing coronavirus contaminated surfaces, especially under low RH. Increased humidity created more favorable conditions for MHV inactivation, resulting in 2.1 log titre reduction. Vaporization with 7.3% hydrogen peroxide presented much better virucidal performance than ozonation in a similar experimental setup, indicating that its application may be more advantageous regarding gaseous disinfection of surfaces contaminated with other coronaviruses, including SARS-CoV-2.

中文翻译：

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在现场条件下，臭氧处理不足以灭活 SARS-CoV-2 替代物


由 SARS-CoV-2 引起的 COVID-19 引发了一场世界性危机，凸显了预防措施在传染病控制中的重要性。 SARS-CoV-2 在表面上的传染性可保持长达数周；因此，需要适当消毒以降低病毒间接传播的风险。气态臭氧处理作为一种易于使用的消毒工具而受到特别关注。在这项研究中，我们评估了在环境条件下（21.8%）和高条件下（49.8-54.2%），气态臭氧处理（>7.3 ppm，2 小时）对 MHV 污染的不锈钢表面和 PBS 悬浮病毒的杀病毒效果。 ）相对湿度。表面病毒被 0.3 g/l BSA 污染。同时，用 7.3% 过氧化氢对受污染的载体进行半小时汽化。获得的结果表明，气态臭氧虽然对悬浮病毒非常有效，但不足以消毒冠状病毒污染的表面，特别是在低相对湿度下。湿度增加为 MHV 灭活创造了更有利的条件，导致滴度降低 2.1 log。在类似的实验装置中，7.3%过氧化氢汽化的杀病毒性能比臭氧化好得多，这表明其应用对于被其他冠状病毒（包括 SARS-CoV-2）污染的表面进行气体消毒可能更有利。