A pharmacological study was conducted to analyze the impact of inhibitors of Na⁺-H⁺ antiporters (amiloride 100 µM) and cation-chloride-cotransporters (bumetanide 200 µM) on two cultivars of the African rice species (Oryza glaberrima Steud) differing in salt resistance (TOG5307: salt-resistant and TOG5949: salt-sensitive) exposed to 75 mM NaCl during 3 days. Amiloride increased Na⁺ accumulation in roots and leaves to a higher extent in salt-resistant TOG5307 than in salt-sensitive TOG5949. Bumetanide reduced Cl⁻ accumulation in both cultivars as well as K⁺ accumulation in TOG5307 and Na⁺ accumulation in TOG5949, suggesting that the cation-chloride-cotransporter in O. glaberrima does not necessarily strictly behave as a Na⁺:K⁺:2Cl⁻ transporter. Inhibitors mainly acted on the absorption step but had low impact on root-to-shoot translocation process. The salt-resistant cultivar TOG5307 was able to efficiently regulate Na⁺ uptake and to cope with high concentration of accumulated toxic ions, as demonstrated by a higher cell viability index and a higher concentration of protein and photosynthetic pigments in NaCl-exposed plants comparatively to salt-sensitive TOG5949.

进行了一项药理学研究，以分析 Na ⁺ -H ⁺逆向转运蛋白抑制剂（阿米洛利 100 µM）和阳离子-氯化物-协同转运蛋白（布美他尼 200 µM）抑制剂对两种不同盐分的非洲水稻品种 ( Oryza glaberrima Steud) 的影响耐受性（TOG5307：耐盐和 TOG5949：盐敏感）在 3 天内暴露于 75 mM NaCl。阿米洛利在耐盐 TOG5307 中比在盐敏感 TOG5949 中增加根和叶中Na ⁺积累的程度更高。布美他尼减少了两个品种中的Cl ^-积累以及TOG5307 和 Na ^{+ 中的}K ⁺积累TOG5949 中的积累，表明O. glaberrima中的阳离子-氯化物-协同转运蛋白不一定严格表现为 Na ⁺ :K ⁺ :2Cl ^-转运蛋白。抑制剂主要作用于吸收步骤，但对根到地上部易位过程的影响较小。耐盐品种 TOG5307 能够有效地调节 Na ⁺吸收并应对高浓度积累的有毒离子，正如与盐相比，暴露于 NaCl 的植物中更高的细胞活力指数和更高浓度的蛋白质和光合色素所证明的敏感的 TOG5949。