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Molecular characterization of a novel mycovirus isolated from Rhizoctonia solani AG-1 IA strain 9-11
Archives of Virology ( IF 2.5 ) Pub Date : 2021-09-15 , DOI: 10.1007/s00705-021-05219-3
Yang Sun 1 , Yanqiong Li 2 , Wenhan Dong 1 , Aili Sun 1 , Ningwei Chen 1 , Zifang Zhao 1 , Yongqing Li 3 , Chengyun Li 1 , Genhua Yang 1
Affiliation  

The complete genome sequence of a double-stranded RNA (dsRNA) virus, Rhizoctonia solani dsRNA virus 11 (RsRV11), isolated from Rhizoctonia solani AG-1 IA strain 9-11 was determined. The RsRV11 genome is 9,555 bp in length and contains three conserved domains: structural maintenance of chromosomes (SMC) superfamily, phosphoribulokinase (PRK), and RNA-dependent RNA polymerase (RdRp). The RsRV11 genome has two non-overlapping open reading frames (ORFs). ORF1 is predicted to encode a 204.12-kDa protein that shares low but significant amino acid sequence similarity with a putative protein encoded by Rhizoctonia solani RNA virus HN008 (RsRV-HN008). ORF2 potentially encodes a 132.41-kDa protein that contains the conserved domain of the RdRp. Phylogenetic analysis indicated that RsRV11 clustered with RsRV-HN008 in a separate clade from other virus families. This implies that RsRV11 and RsRV-HN008 should be included in a new mycovirus taxon close to the family Megabirnaviridae and that RsRV11 is a new mycovirus.



中文翻译:

从立枯丝核菌 AG-1 IA 菌株 9-11 中分离的新型真菌病毒的分子特征

立枯丝核菌中分离的双链 RNA (dsRNA) 病毒,立枯丝核菌 dsRNA 病毒 11 (RsRV11) 的完整基因组序列确定了 AG-1 IA 菌株 9-11。RsRV11 基因组长度为 9,555 bp,包含三个保守结构域:染色体结构维持 (SMC) 超家族、磷酸核糖激酶 (PRK) 和 RNA 依赖性 RNA 聚合酶 (RdRp)。RsRV11 基因组有两个不重叠的开放阅读框 (ORF)。预计 ORF1 将编码一种 204.12-kDa 的蛋白质,该蛋白质与由立枯丝核菌 RNA 病毒 HN008 (RsRV-HN008) 编码的推定蛋白质具有低但显着的氨基酸序列相似性。ORF2 可能编码一个 132.41-kDa 的蛋白质,该蛋白质包含 RdRp 的保守结构域。系统发育分析表明,RsRV11 与 RsRV-HN008 聚集在与其他病毒家族不同的进化枝中。这意味着 RsRV11 和 RsRV-HN008 应包含在与该家族关系密切的新真菌病毒分类群中Megabirnaviridae和 RsRV11 是一种新的真菌病毒。

更新日期:2021-10-07
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