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Molecular characterization of a novel mycovirus isolated from Rhizoctonia solani AG-1 IA strain 9-11

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Abstract

The complete genome sequence of a double-stranded RNA (dsRNA) virus, Rhizoctonia solani dsRNA virus 11 (RsRV11), isolated from Rhizoctonia solani AG-1 IA strain 9-11 was determined. The RsRV11 genome is 9,555 bp in length and contains three conserved domains: structural maintenance of chromosomes (SMC) superfamily, phosphoribulokinase (PRK), and RNA-dependent RNA polymerase (RdRp). The RsRV11 genome has two non-overlapping open reading frames (ORFs). ORF1 is predicted to encode a 204.12-kDa protein that shares low but significant amino acid sequence similarity with a putative protein encoded by Rhizoctonia solani RNA virus HN008 (RsRV-HN008). ORF2 potentially encodes a 132.41-kDa protein that contains the conserved domain of the RdRp. Phylogenetic analysis indicated that RsRV11 clustered with RsRV-HN008 in a separate clade from other virus families. This implies that RsRV11 and RsRV-HN008 should be included in a new mycovirus taxon close to the family Megabirnaviridae and that RsRV11 is a new mycovirus.

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Acknowledgements

This research was supported the National Natural Scientific Foundation of China (31960525, 31160352, 31360423) and grants-in-aid from the Scientific Research Project of Kunming University (YJL16006) and the General Program of University Union in Yunnan Province (2018FH 001-032).

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Authors and Affiliations

  1. State Key Laboratory for Protection and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Yang Sun, Wenhan Dong, Aili Sun, Ningwei Chen, Zifang Zhao, Chengyun Li & Genhua Yang

  2. College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, Yunnan, China

    Yanqiong Li

  3. Zhaotong Plant Protection and Quarantine Station, Zhaotong, 657000, China

    Yongqing Li

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  1. Yang Sun
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  2. Yanqiong Li
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Correspondence to Genhua Yang.

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Sun, Y., Li, Y., Dong, W. et al. Molecular characterization of a novel mycovirus isolated from Rhizoctonia solani AG-1 IA strain 9-11. Arch Virol 166, 3229–3232 (2021). https://doi.org/10.1007/s00705-021-05219-3

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  • DOI: https://doi.org/10.1007/s00705-021-05219-3

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