Our study attempted to explore the mechanism underlying the role of LuxR family transcriptional regulator abaR in biofilm formation by Acinetobacter baumannii. The abaR gene was knocked out in ATCC 17978 strain using homologous recombination method. The growth curve and biofilm formation were measured in the wild type and abaR gene knockdown strains. Transcriptome sequencing was performed in the wild type and abaR gene knockdown strains following 8 h of culture. The growth curve in the abaR gene knockdown strain was similar to that of the wild-type strain. Biofilm formation significantly declined in the abaR gene knockdown strain at 8 and 48 h after culture. A total of 137 differentially expressed genes (DEGs) were obtained including 20 downregulated DEGs and 117 upregulated DEGs. Genes with differential expression were closely related to viral procapsid maturation (GO:0046797), acetoin catabolism (GO:0045150), carbon metabolism (ko01200), and the glycolysis/gluconeogenesis (ko00010)-related pathways. The results of the eight verified expression DEGs were consistent with the results predicted by bioinformatics. AbaR gene knockdown significantly affected biofilm formation by A. baumannii ATCC 17978 strain. The glycolysis/gluconeogenesis pathways were significantly dysregulated and induced by abaR gene knockdown in A. baumannii.

我们的研究试图探索 LuxR 家族转录调节因子abaR在鲍曼不动杆菌生物膜形成中的作用机制。ATCC 17978菌株采用同源重组方法敲除abaR基因。在野生型和abaR基因敲低菌株中测量生长曲线和生物膜形成。在培养 8 小时后，在野生型和abaR基因敲低菌株中进行转录组测序。abaR基因敲低菌株的生长曲线与野生型菌株相似。abaR中的生物膜形成显着下降培养后8和48小时的基因敲低菌株。共获得137个差异表达基因（DEGs），包括20个下调的DEGs和117个上调的DEGs。具有差异表达的基因与病毒前衣壳成熟 (GO:0046797)、乙偶姻分解代谢 (GO:0045150)、碳代谢 (ko01200) 和糖酵解/糖异生 (ko00010) 相关途径密切相关。8个经过验证的表达DEG的结果与生物信息学预测的结果一致。AbaR基因敲低显着影响鲍曼不动杆菌ATCC 17978 菌株的生物膜形成。鲍曼不动杆菌中abaR基因敲低导致糖酵解/糖异生途径显着失调和诱导。