As a valuable medicinal plant, Salvia plebeia R. Brown (S. plebeia) belongs to the Lamiaceae family that has been subjected to over-exploitation in its natural habitat for phytochemical and pharmacological studies. The present study focuses on the development of a micropropagation protocol for sustainable propagation and conservation of S. plebeia. Direct organogenesis (from shoot tips and cotyledonary nodes explants) and globular bodies (GBs) induction (from hypocotyl explants) systems have been established in S. plebeia. Alternative collection methods need to be developed for the large-scale propagation of S. plebeia. In addition, roots as explants can also induce adventitious shoots via callus. The highest and number of regenerated shoots (7.0 ± 0.8) per shoot tips was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 0.1 mg L⁻¹ indole-3-acetic acid (IAA) and 1.0 mg L⁻¹ 6-benzyladenine (6-BA), the proliferation of shoots and shoots rooted were carried out on the same medium treatments almost synchronously. Similarly, MS medium supplemented with 0.1 mg L⁻¹ IAA and 1.0 mg L⁻¹ thidiazuron (TDZ) yielded the maximum number of shoots (37.5 ± 1.3) with 100% shoot sprouting frequency. Simultaneously, a protocol was developed for GB induction from hypocotyl explants, and it produced 17.4 GBs per explant with 82.7% response on MS medium supplemented with TDZ (1.0 mg L⁻¹) and IAA (0.1 mg L⁻¹), and produced GBs were characterized by globular, heart-shaped, and cotyledonary stages and successfully germinated on hormone-free MS medium, the developmental process of which was similar to that embryo. The acclimatized plantlets with well-developed root systems were successfully shifted to the natural soils with a 100% survival rate. Taken together, it is the foremost report on in vitro regeneration of S. plebeia; this work will facilitate in germplasm preservation and genetic transformation and provides necessary plant materials for various biotechnological and pharmaceutical applications.

Salvia plebeia R. Brown ( S. plebeia )作为一种珍贵的药用植物，属于唇形科植物，在其天然栖息地进行植物化学和药理研究时已被过度开发。本研究的重点是为S. plebeia 的可持续繁殖和保护制定微繁殖协议。已经在S. plebeia 中建立了直接器官发生（来自芽尖和子叶节点外植体）和球状体（GB）诱导（来自下胚轴外植体）系统。需要为S. plebeia的大规模繁殖开发替代收集方法。此外，作为外植体的根也可以通过愈伤组织诱导不定芽。在补充有 0.1 mg L ^-1吲哚-3-乙酸 (IAA) 和 1.0 mg L ^-1的组合的 Murashige 和 Skoog (MS) 培养基上获得最高和每个芽尖的再生芽数 (7.0 ± 0.8) 6-苄基腺嘌呤（6-BA），芽和生根的增殖在相同的培养基上几乎同步进行。类似地，添加了 0.1 mg L ^-1 IAA 和 1.0 mg L ^{-1 的}MS 培养基噻二唑隆 (TDZ) 产生最大数量的芽 (37.5 ± 1.3)，芽发芽频率为 100%。同时，开发了一种用于从下胚轴外植体诱导 GB 的方案，它在补充有 TDZ (1.0 mg L ^-1 ) 和 IAA (0.1 mg L ^-1 ) 的MS 培养基上产生了 17.4 GBs 每个外植体的响应率为 82.7% ，并产生了 GBs具有球状、心形和子叶阶段的特征，并在无激素的 MS 培养基上成功发芽，其发育过程与胚胎相似。根系发达的驯化苗成功转移到天然土壤中，成活率100%。综上所述，这是关于S. plebeia体外再生的最重要报告; 这项工作将促进种质保存和遗传转化，并为各种生物技术和药物应用提供必要的植物材料。