Abstract
As a valuable medicinal plant, Salvia plebeia R. Brown (S. plebeia) belongs to the Lamiaceae family that has been subjected to over-exploitation in its natural habitat for phytochemical and pharmacological studies. The present study focuses on the development of a micropropagation protocol for sustainable propagation and conservation of S. plebeia. Direct organogenesis (from shoot tips and cotyledonary nodes explants) and globular bodies (GBs) induction (from hypocotyl explants) systems have been established in S. plebeia. Alternative collection methods need to be developed for the large-scale propagation of S. plebeia. In addition, roots as explants can also induce adventitious shoots via callus. The highest and number of regenerated shoots (7.0 ± 0.8) per shoot tips was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 0.1 mg L−1 indole-3-acetic acid (IAA) and 1.0 mg L−1 6-benzyladenine (6-BA), the proliferation of shoots and shoots rooted were carried out on the same medium treatments almost synchronously. Similarly, MS medium supplemented with 0.1 mg L−1 IAA and 1.0 mg L−1 thidiazuron (TDZ) yielded the maximum number of shoots (37.5 ± 1.3) with 100% shoot sprouting frequency. Simultaneously, a protocol was developed for GB induction from hypocotyl explants, and it produced 17.4 GBs per explant with 82.7% response on MS medium supplemented with TDZ (1.0 mg L−1) and IAA (0.1 mg L−1), and produced GBs were characterized by globular, heart-shaped, and cotyledonary stages and successfully germinated on hormone-free MS medium, the developmental process of which was similar to that embryo. The acclimatized plantlets with well-developed root systems were successfully shifted to the natural soils with a 100% survival rate. Taken together, it is the foremost report on in vitro regeneration of S. plebeia; this work will facilitate in germplasm preservation and genetic transformation and provides necessary plant materials for various biotechnological and pharmaceutical applications.
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Funding
The work was supported by the National Natural Science Foundation of China (31972258, 32071747) and the Applied Basic Project of the Sichuan Science and Technology Department (2018JY0192).
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LJZ designed and guided the experiments. QGW and CZ performed the experiments, analyzed the data and wrote the article. HLY, YXS, and JYH made substantial contributions to the data analysis and the manuscript revision. All authors read and approved the final manuscript.
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Wu, Q., Zhang, C., Yang, H. et al. In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant. In Vitro Cell.Dev.Biol.-Plant 58, 51–60 (2022). https://doi.org/10.1007/s11627-021-10223-y
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DOI: https://doi.org/10.1007/s11627-021-10223-y