Abstract

2,3,7,8,12,13,17,18-Octaethyl-21H,23H-porphine (OEP) was successfully radiolabeled with technetium-99m by direct technique using two different reducing agents: stannous chloride and sodium borohydride. High radiochemical yield (97%) and in vitro stability for up to 8 h were obtained using sodium borohydride. Paper chromatography (PC), paper electrophoresis, and high-performance liquid chromatography (HPLC) were used to evaluate the radiochemical yield and identify the purity of the final product. Biodistribution studies of the ^99mTc-OEP complex (prepared using sodium borohydride) in tumor-bearing mice showed high target/nontarget (T, right thigh/NT, left thigh) ratio of 4.40 ± 0.12 at 60 min post injection, suggesting that this complex can be used as a solid tumor imaging agent.

中文翻译：

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氯化亚锡和硼氢化钠作为还原剂对 2,3,7,8,12,13,17,18-Octaethyl-21H,23H-Porphine 与锝-99m 进行肿瘤成像放射性标记的比较研究

摘要

2,3,7,8,12,13,17,18-Octaethyl-21 H ,23 H -porphine (OEP) 被锝 99 m通过直接技术成功放射性标记，使用两种不同的还原剂：氯化亚锡和硼氢化钠. 使用硼氢化钠可获得高放射化学产率 (97%) 和长达 8 小时的体外稳定性。纸色谱 (PC)、纸电泳和高效液相色谱 (HPLC) 用于评估放射化学产率并确定最终产品的纯度。^{99 m 的}生物分布研究Tc-OEP 复合物（使用硼氢化钠制备）在荷瘤小鼠体内显示出高靶点/非靶点（T，右大腿/NT，左大腿）比在注射后 60 分钟达到 4.40 ± 0.12，表明该复合物可用作一种实体瘤显像剂。