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A Comparative Study of Stannous Chloride and Sodium Borohydride as Reducing Agents for the Radiolabeling of 2,3,7,8,12,13,17,18-Octaethyl-21H,23H-Porphine with Technetium-99m for Tumor Imaging

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Radiochemistry Aims and scope

Abstract

2,3,7,8,12,13,17,18-Octaethyl-21H,23H-porphine (OEP) was successfully radiolabeled with technetium-99m by direct technique using two different reducing agents: stannous chloride and sodium borohydride. High radiochemical yield (97%) and in vitro stability for up to 8 h were obtained using sodium borohydride. Paper chromatography (PC), paper electrophoresis, and high-performance liquid chromatography (HPLC) were used to evaluate the radiochemical yield and identify the purity of the final product. Biodistribution studies of the 99mTc-OEP complex (prepared using sodium borohydride) in tumor-bearing mice showed high target/nontarget (T, right thigh/NT, left thigh) ratio of 4.40 ± 0.12 at 60 min post injection, suggesting that this complex can be used as a solid tumor imaging agent.

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REFERENCES

  1. Jurisson, S., Bering, D., Jia, W., and Ma, D., Chem. Rev., 1993, vol. 93, pp. 1137–1156.

    Article  CAS  Google Scholar 

  2. Jurisson, S. and Lydon, J., Chem. Rev., 1999, vol. 99, pp. 2205–2218.

    Article  CAS  Google Scholar 

  3. Xu, Y., Luo, S., Pan, D., Wang, L., Zhou, Y., and Yang, M., J. Radioanal. Nucl. Chem., 2012, vol. 295, pp. 1861–1866.

    Google Scholar 

  4. Deutsch, E., Libson, K., and Jurisson, S., Prog. Inorg. Chem., 1983, vol. 30, pp. 73–139.

    Google Scholar 

  5. Sanad, M.H., Saleh, G.M., and Marzook, F.A., J. Label. Compd. Radiopharm., 2017, vol. 60, pp. 600–607.

    Article  CAS  Google Scholar 

  6. Kessel, D., Photodiagn. Photodyn. Ther., 2004, vol. 1, pp. 3–7.

    Article  CAS  Google Scholar 

  7. Allison, R., Downie, G., Cuenca, R., Hu, X., Childs, C., and Sibata, C., Photodiagn. Photodyn. Ther., 2004, vol. 1, pp. 27–42.

    Article  CAS  Google Scholar 

  8. Ko, Y., Yun, K., Kang, M., Park, J., Lee, K., Park, S., and Shin, J., Bioorg. Med. Chem. Lett., 2007, vol. 17, no. 10, pp. 2789–2794.

    Article  CAS  Google Scholar 

  9. Kolarova, H., Macecek, J., Nevrelova, P., Huf, M., Tomecka, M., Bajgar, R., Mosinger, J., and Strnad, M., Toxicol. in Vitro, 2005, vol. 19, pp. 971–974.

    Article  CAS  Google Scholar 

  10. Das, T., Chakraborty, S., Sarma, H., Banerjee, S., and Venakatesh, M., Nucl. Med. Biol., 2010, vol. 37, no. 5, pp. 655–663.

    Article  CAS  Google Scholar 

  11. Banerjee, S., Das, T., Samuel, G., Sarma, H., Venkatesh, M., and Pillai, M., Nucl. Med. Commun., 2001, vol. 22, pp. 1101–1107.

    Article  CAS  Google Scholar 

  12. Bonnett, R., White, R., Winfield, U., and Berenbaum, M., Biochem. J., 1989, vol. 261, no. 1, pp. 277–280.

    Article  CAS  Google Scholar 

  13. Hambright, P., Fawwaz, R., Valk, P., et al, Bioinorg. Chem., 1975, vol. 5, pp. 87–92.

    Article  CAS  Google Scholar 

  14. Zanelli, G. and Kaelin, A., Brit. J. Radiol., 1981, vol. 54, pp. 403.

    Article  CAS  Google Scholar 

  15. Whelan, H., Kras, L., Ozker, K., et al., J. Neurooncol., 1994, vol. 22, pp. 7–13.

    Article  CAS  Google Scholar 

  16. Bhalgat, M., Roberts, J., Mercer-Smith, J., et al., Nucl. Med. Biol., 1997, vol. 24, pp. 179–185.

    Article  CAS  Google Scholar 

  17. Subbarayan, M., Shetty, S., Srivastava, T., et al., J. Porphyrins Phthalocyanines, 2001, vol. 5, p. 824.

    Article  CAS  Google Scholar 

  18. Kavali, R., Lee, B., Moon, B., et al., J. Label. Compd. Radiopharm., 2005, vol. 48, p. 749.

    Article  CAS  Google Scholar 

  19. Jia, Z., Deng, H., and Pu, M., Nucl. Med. Biol., 2007, vol. 34, pp. 643.

    Article  CAS  Google Scholar 

  20. Fazaeli, Y., Jalilian, A., Amini, M., Khosro, A., Rahiminejad, A., Bolourinovin, F., Moradkhani, S., and Majdabadi, A., Nucl. Med. Mol. Imaging, 2012, vol. 46, no. 1, pp. 20–26.

    Article  CAS  Google Scholar 

  21. Murugesa, S., Shetty, S., Srivastava, T., Noronha, O., and Samuel, A., Appl. Radiat. Isot., 2001, vol. 55, no. 5, pp. 641–646.

    Article  CAS  Google Scholar 

  22. Shetty, S., Murugesan, S., Chatterjee, S., Banerjee, S., Srivastava, T., Noronha, O., and Samuel, A., J. Label. Compd. Radiopharm., 1996, vol. 38, pp. 411–418.

    Article  CAS  Google Scholar 

  23. Chatterjee, S., Murugesan, S., Kamat, J., Shetty, S., Srivastava, T., Noronha, O., Samuel, A., and Devasagayam, T., Arch. Biochem. Biophys., 1997, vol. 339, pp. 242–249.

    Article  CAS  Google Scholar 

  24. Sanad, M.H. and Challan, S.B., Radiochemistry, 2017, vol. 59, pp. 307-312. https://doi.org/10.1134/S1066362217030158

    Article  CAS  Google Scholar 

  25. Sanad, M.H. and Emad, H.B., Radiochim. Acta, 2015, vol. 103(12), pp. 879–891.

    Article  CAS  Google Scholar 

  26. Sanad, M.H. and El-Tawoosy, M., Radioanalytical and Nuclear Chemistry, 2013 vol. 298(2), pp. 1105-1109.

    Article  Google Scholar 

  27. Amin, A.M., Sanad, M.H., and Abd-Elhaliem, S.M., Radiochemistry, 2013, vol. 55, pp. 624-628. https://doi.org/10.1134/S1066362213060118

    Article  CAS  Google Scholar 

  28. Marzook, E.A., Talaat, H.M., and Challan, S.B., Radiochemistry, 2018, vol. 60, no. 3, pp. 309–315. https://doi.org/10.1134/S1066362218030141

    Article  CAS  Google Scholar 

  29. Mohini, G., Tapas, D., Haladhar, D.S., and Sharmila, B., J. Radioanal. Nucl. Chem., 2016, vol. 307, pp. 1537–1544.

    Article  Google Scholar 

  30. Sanad, M.H., Salama, D.H., and Marzook, F.A., Radiochim. Acta, 2017, vol. 105, p. 389.

    Article  CAS  Google Scholar 

  31. Sanad, M.H. and Ibrahim, I.T., Radiochemistry, 2015, vol. 57, pp. 425–430. https://doi.org/10.1134/S1066362215040165

    Article  CAS  Google Scholar 

  32. Borai, E., Sanad, M.H., and Fouzy, A.S.M., Radiochemistry, 2016, vol. 58, no. 1, pp. 84–91. https://doi.org/10.1134/S1066362216010136

    Article  CAS  Google Scholar 

  33. Sanad, M.H., Talaat, H.M., Radiochemistry, 2017, vol. 59, pp. 396-401.

    Article  CAS  Google Scholar 

  34. Ebtisam, A.M., Ahmed, S.E., and Fawzy, A.M., Journal of Radiation Research and Applied Sciences, 2019, vol. 12(1), pp. 304–310.

    Article  Google Scholar 

  35. Faheem, A.R., Bokhari, T.H., Roohi, S., Mushtaq, A., and Sohaib, M., J. Nucl. Med. Biol., 2013, vol. 40, p. 148.

    Article  CAS  Google Scholar 

  36. Faheem, A.R., Syed, A.R.N., Muhammad, M., Samina, R., Ameer, F.Z., Zulfi qar, A.K., and Rashid, R., J. Chil. Chem.Soc., 2017, vol. 62, p. 3345.

    Article  Google Scholar 

  37. Saleha, T., Syed, F. A. R., and Ummar, A., Biomed. J. Sci. Tech. Res., 2018, vol. 1, pp. 5787–5796. https://doi.org/10.26717/BJSTR.2018.07.001475

    Article  Google Scholar 

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ACKNOWLEDGMENTS

This work was supported by STDF – National Research Centre under project number 41535 – PI Ahmed Sayed Morsy Fouzy.

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Authors and Affiliations

  1. Labeled Compounds Department, Hot Laboratories Center, Egyptian Atomic Energy Authority, POB13759, Cairo, Egypt

    M. H. Sanad, M. A. Gizawy, M. A. Motaleb & I. T. Ibrahim

  2. Chemistry Department, Faculty of Science, Ain Shams University, POB11566, Cairo, Egypt

    E. A. Saad

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  1. M. H. Sanad
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  2. M. A. Gizawy
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  3. M. A. Motaleb
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Correspondence to M. H. Sanad.

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Sanad, M.H., Gizawy, M.A., Motaleb, M.A. et al. A Comparative Study of Stannous Chloride and Sodium Borohydride as Reducing Agents for the Radiolabeling of 2,3,7,8,12,13,17,18-Octaethyl-21H,23H-Porphine with Technetium-99m for Tumor Imaging. Radiochemistry 63, 512–519 (2021). https://doi.org/10.1134/S1066362221040159

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