Abstract

The influence of humic substances (HSs) of coals (HS-cl), shale (HS-sl), and mud (HS-md) isolated in Mongolia and silver nanocomposites (NCs) synthesized on their basis on the viability of Rhodococcus erythropolis (gram positive bacteria) and Pseudomonas oryzihabitans (gram negative bacteria) is investigated using methods for measuring the optical density of a bacterial suspension, diffusion into agar, as well as studying the intensity of biofilm formation. It is shown that the effects of HSs and NCs on bacteria in dynamics are different in R. erythropolis and P. oryzihabitans. HS-sl stimulated the growth of R. erythropolis on the first day of observation, and in P. oryzihabitans this effect was noted later, at 2 days of the experiment. Despite the absence of bacteriostatic effects for HSs, a decrease in biofilm formation of bacteria under their influence has been revealed. HS-cl and HS-md reduced the biofilm formation of R. erythropolis, and HS-sl reduced P. oryzihabitans. The addition of HSs to HCs retains the bactericidal effect of NC HS-cl/Ag and NC HS-md/Ag with respect to R. erythropolis. A decrease in biofilm formation of gram-negative bacteria P. oryzihabitans occurred under the influence of NC HS-md/Ag. NC HS-cl/Ag, in contrast, enhanced the reproduction of P. oryzihabitans throughout the observation period. Thus, the reaction of microorganisms to the effects of HSs in the composition of NCs depends on the morphology of the bacteria themselves, as well as on the chemical structure and functional composition of the active substances. The obtained data and previously published results on the positive effect of silver NCs and HSs on plants open the prospects for further investigation of the effects of using NCs of HSs when introduced into the soil in combination with rhizospheric bacteria to increase the resistance of cultivated plants to stress factors.

中文翻译：

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腐殖质和腐殖质基银纳米复合材料对根际微生物生存力的影响

摘要

蒙古分离出的煤 (HS-cl)、页岩 (HS-sl) 和泥浆 (HS-md) 的腐殖质 (HSs) 以及在其基础上合成的银纳米复合材料 (NCs) 对红球菌( Rhodococcus erythropolis) 的影响。革兰氏阳性菌）和米氏假单胞菌（革兰氏阴性菌）使用测量细菌悬浮液的光密度、扩散到琼脂中的方法以及研究生物膜形成的强度的方法进行研究。结果表明，HSs和NCs对细菌的动力学影响在R. erythropolis和P. oryzihabitans中是不同的。HS-sl在观察的第一天刺激了R. erythropolis和P. oryzihabitans 的生长这种效果后来在实验的第 2 天被注意到。尽管对 HS 没有抑菌作用，但已发现在其影响下细菌的生物膜形成减少。HS-cl 和 HS-md 减少了R. erythropolis的生物膜形成，而 HS-sl 减少了P. oryzihabitans。向 HC 中添加 HS 保留了 NC HS-cl/Ag 和 NC HS-md/Ag 对R. erythropolis的杀菌作用。在NC HS-md/Ag 的影响下，革兰氏阴性菌P. oryzihabitans 的生物膜形成减少。相反，NC HS-cl/Ag 增强了P. oryzihabitans的繁殖在整个观察期内。因此，微生物对 HSs 在 NCs 组成中的作用的反应取决于细菌本身的形态，以及活性物质的化学结构和功能组成。获得的数据和先前发表的关于银 NCs 和 HSs 对植物的积极影响的结果为进一步研究使用 HSs 的 NCs 与根际细菌结合使用以增加栽培植物的抗性的效果开辟了前景。压力因素。