Background

The aim of this study is to investigate of the relationship between GSTM1 gene variations and serum trace elements, plasma malondialdehyde levels in patient with colorectal cancer.

Mateials and Methods.

Genotype distributions of GSTM1 gene variations were determined using real-time polymerase chain reaction method. Serum trace element levels were determined using atomic absorption spectrophotometer method and plasma MDA levels were measurement by spectrophotometric method.

Results

Serum Cu levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GA heterozygous genotype of the GSTM1 (rs 112,778,559) gene variation compared to healthy controls (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in patients carrying GG homozygous genotype of the GSTM1 (rs 112778559) gene variation compared to healthy controls carrying same genotype (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GG homozygous genotype of the GSTM1 (rs 12068997) gene variation compared to healthy controls (p < 0.05). On the other hand, serum Se levels were detected significantly lower in CRC patients carrying GA heterozygous and GG homozygous genotypes for GSTM1 (rs 112,778,559) and (rs 12,068,997) gene variations compared to healthy controls (p < 0.05).

Conclusion

In our study, the evaluation of serum Cu, Zn and Se trace element levels and plasma MDA levels according to GSTM1 gene variations genotype distributions were enabled to obtain important biomarkers in terms of CRC development and progression.

中文翻译：

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大肠癌患者GSTM1基因变异与血清微量元素、血浆丙二醛水平的关系研究

背景

本研究旨在探讨结直肠癌患者GSTM1基因变异与血清微量元素、血浆丙二醛水平的关系。

材料和方法。

使用实时聚合酶链反应方法确定 GSTM1 基因变异的基因型分布。血清微量元素含量采用原子吸收分光光度计法测定，血浆MDA含量采用分光光度法测定。

结果

与健康对照组相比，携带 GSTM1 GA 杂合基因型 (rs 112,778,559) 基因变异的 CRC 患者组的血清铜水平、血浆 MDA 水平和铜/锌比率显着高于健康对照组 (p < 0.05)。与携带相同基因型的健康对照组相比，携带 GSTM1 (rs 112778559) 基因变异的 GG 纯合基因型患者的血清铜、锌水平、血浆 MDA 水平和铜/锌比率显着升高 (p < 0.05)。与健康对照组相比，携带 GSTM1 (rs 12068997) 基因变异的 GG 纯合基因型的 CRC 患者组的血清铜、锌水平、血浆 MDA 水平和铜/锌比率显着高于健康对照组 (p < 0.05)。另一方面，

结论

在我们的研究中，根据 GSTM1 基因变异基因型分布评估血清 Cu、Zn 和 Se 微量元素水平和血浆 MDA 水平能够获得关于 CRC 发展和进展的重要生物标志物。