Abstract
Background
The aim of this study is to investigate of the relationship between GSTM1 gene variations and serum trace elements, plasma malondialdehyde levels in patient with colorectal cancer.
Mateials and Methods.
Genotype distributions of GSTM1 gene variations were determined using real-time polymerase chain reaction method. Serum trace element levels were determined using atomic absorption spectrophotometer method and plasma MDA levels were measurement by spectrophotometric method.
Results
Serum Cu levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GA heterozygous genotype of the GSTM1 (rs 112,778,559) gene variation compared to healthy controls (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in patients carrying GG homozygous genotype of the GSTM1 (rs 112778559) gene variation compared to healthy controls carrying same genotype (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GG homozygous genotype of the GSTM1 (rs 12068997) gene variation compared to healthy controls (p < 0.05). On the other hand, serum Se levels were detected significantly lower in CRC patients carrying GA heterozygous and GG homozygous genotypes for GSTM1 (rs 112,778,559) and (rs 12,068,997) gene variations compared to healthy controls (p < 0.05).
Conclusion
In our study, the evaluation of serum Cu, Zn and Se trace element levels and plasma MDA levels according to GSTM1 gene variations genotype distributions were enabled to obtain important biomarkers in terms of CRC development and progression.
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Acknowledgements
Our study was carried out in Trakya University Faculty of Medicine, Department of Biophysics, Department of Medical Oncology and Department of Radiation Oncology.
Funding
This study was supported by the Trakya University Scientific Research Projects Unit (TUBAP) with the project number 2013/14.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [Arzu Ay], [Nevra Alkanli], [Tevfik Gulyasar], [Tammam Sipahi], [Irfan Cicin], [Zafer Kocak] and [Necdet Sut]. The first draft of the manuscript was written by [Arzu Ay and Nevra Alkanli] and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript. The corresponding author attests that all listed authors meet the authorship criteria and that no other authors meeting the criteria have been omitted.
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For our study, it was applied for Trakya University Faculty of Medicine Non-Invasive Clinical Research Ethics Committee and ethics committee approval was obtained with the protocol code of TÜTF-GOKAEK 2012/198.
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Signed informed consent form was collected from each individual in the patient group with CRC and healthy control group
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Ay, A., Gulyasar, T., Alkanli, N. et al. Investigation of the relationship between GSTM1 gene variations and serum trace elements, plasma malondialdehyde levels in patients with colorectal cancer. Mol Biol Rep 48, 6911–6921 (2021). https://doi.org/10.1007/s11033-021-06694-2
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DOI: https://doi.org/10.1007/s11033-021-06694-2