Biological pretreatment was investigated to increase ethanol production from lignocellulosic biomass, like sugarcane bagasse. Enzyme secretion, changes in substrate composition, enzymatic hydrolysis, and ethanol yield after pretreatment by different basidiomycetes were evaluated. Analysis by Fourier transform infrared spectroscopy showed that Pleurotus pulmonarius PS2001 and Trametes villosa 82I6 promoted more extensive selective modifications in the lignin content. Glucose release during enzymatic hydrolysis of samples pretreated with P. pulmonarius PS2001 for 35, 42, and 49 days and with T. villosa 82I6 for 21, 28, and 49 days were higher than the control (48.5 ± 2.38 mg/g), i.e., 68.4 ± 0.7, 76.3 ± 1.6, and 76.5 ± 2.1 mg/g and 70.9 ± 8.3, 77.8 ± 5.8, and 77.6 ± 4.2 mg/g, respectively. During the fermentation of hydrolysates of samples pretreated with T. villosa 82I6 for 14, 28, and 49 days, a maximum ethanol yield of 17.8 ± 1.9, 19.1 ± 2.8, and 20.2 ± 0.5 mg/g, respectively, was achieved. A positive effect of biological pretreatment on hydrolysis and fermentation was demonstrated.

研究了生物预处理以增加木质纤维素生物质（如甘蔗渣）的乙醇产量。评估了酶分泌、底物组成的变化、酶水解和不同担子菌预处理后的乙醇产量。傅里叶变换红外光谱分析表明，Pleurotus pulmonarius PS2001 和Trametes villosa 82I6 促进了木质素含量的更广泛的选择性修饰。用P预处理的样品在酶促水解过程中的葡萄糖释放。pulmonarius PS2001 持续 35、42 和 49 天，并且T . 比略萨21、28 和 49 天的 82I6 高于对照 (48.5 ± 2.38 mg/g)，即 68.4 ± 0.7、76.3 ± 1.6 和 76.5 ± 2.1 mg/g 和 70.9 ± 8.3、77.8 和 ±分别为 77.6 ± 4.2 毫克/克。在用T预处理的样品的水解物发酵过程中。villosa 82I6 14、28 和 49 天，分别达到 17.8 ± 1.9、19.1 ± 2.8 和 20.2 ± 0.5 mg/g 的最大乙醇产量。证明了生物预处理对水解和发酵的积极影响。