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Fungal pretreatment of sugarcane bagasse: a green pathway to improve saccharification and ethanol production

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Abstract

Biological pretreatment was investigated to increase ethanol production from lignocellulosic biomass, like sugarcane bagasse. Enzyme secretion, changes in substrate composition, enzymatic hydrolysis, and ethanol yield after pretreatment by different basidiomycetes were evaluated. Analysis by Fourier transform infrared spectroscopy showed that Pleurotus pulmonarius PS2001 and Trametes villosa 82I6 promoted more extensive selective modifications in the lignin content. Glucose release during enzymatic hydrolysis of samples pretreated with P. pulmonarius PS2001 for 35, 42, and 49 days and with T. villosa 82I6 for 21, 28, and 49 days were higher than the control (48.5 ± 2.38 mg/g), i.e., 68.4 ± 0.7, 76.3 ± 1.6, and 76.5 ± 2.1 mg/g and 70.9 ± 8.3, 77.8 ± 5.8, and 77.6 ± 4.2 mg/g, respectively. During the fermentation of hydrolysates of samples pretreated with T. villosa 82I6 for 14, 28, and 49 days, a maximum ethanol yield of 17.8 ± 1.9, 19.1 ± 2.8, and 20.2 ± 0.5 mg/g, respectively, was achieved. A positive effect of biological pretreatment on hydrolysis and fermentation was demonstrated.

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Data availability

Data available on request from the authors: the data that support the finding of this study are available from the corresponding author, on request.

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Acknowledgements

We would like to thank Dr. Luiz Humberto Gomes of Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo, Piracicaba, SP for the kind donation of Saccharomyces cerevisiae strain CAT-1 and Professor George Jackson de Moraes Rocha for the kind delivery of sugarcane bagasse.

Funding

This research was supported by grants from CAPES (3255/2013) and the National Council for Scientific and Technological Development (CNPq) (472153/2013–7). Marli Camassola is a recipient of a CNPq Research Fellowship.

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Authors and Affiliations

  1. Laboratório de Enzimas e Biomassas, Universidade de Caxias do Sul, Rua Francisco Getúlio Vargas 1130, Caxias do Sul, RS, 95070-560, Brazil

    Caroline Hartmann, Roselei Claudete Fontana & Marli Camassola

  2. Empresa Brasileira de Pesquisa AgropecuáriaEmbrapa Agroenergia, Parque Estação Biológica - PqEB s/n°, Brasília, DF, 70770-901, Brazil

    Félix Gonçalves de Siqueira

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  1. Caroline Hartmann
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  2. Roselei Claudete Fontana
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  3. Félix Gonçalves de Siqueira
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Contributions

Conceptualization and design, MC; methodology, CH, RCF, and MC; investigation, CH and RCF; writing-original draft preparation, CH and MC; writing-review and editing, CH, FGS, and MC; administrative and technical support, and obtaining of funding: MC.

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Correspondence to Marli Camassola.

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Hartmann, C., Fontana, R.C., de Siqueira, F.G. et al. Fungal pretreatment of sugarcane bagasse: a green pathway to improve saccharification and ethanol production. Bioenerg. Res. 15, 1130–1143 (2022). https://doi.org/10.1007/s12155-021-10329-6

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