The purpose of the current investigation is to standardize the aqueous leaf extract of Coriandrum sativum L. with a high-performance thin-layer chromatography (HPTLC) method. The method involves the determination and validation of extract with quercetin as standard. Chromatography was performed on pre-coated silica gel G 60 F₂₅₄ plate as the stationary phase with formic acid, ethyl acetate and toluene as the mobile phase in the ratio 1:4:5 (V/V). Identification and quantification were conducted optical densitometrically at 254 nm. The R_F value of quercetin obtained for the standard as well as the leaf extract was found to be 0.50. The method was validated for intra-day and inter-day precision and accuracy. The accuracy of the method was established by a recovery study, with 98.04 ± 0.21% average recovery of quercetin. The lyophilized coriander extract samples contained 0.058 ± 0.02% quercetin. The HPTLC method resulted in the development of an accurate, precise, reproducible and pragmatic approach to standardize the aqueous leaf extract of C. sativum L.

当前调查的目的是使用高效薄层色谱 (HPTLC) 方法对芫荽叶提取物进行标准化。该方法涉及以槲皮素为标准的提取物的测定和验证。色谱在预涂硅胶 G 60 F ₂₅₄板上作为固定相，以甲酸、乙酸乙酯和甲苯为流动相，以 1:4:5 ( V/V )的比例进行。在 254 nm 处以光密度计进行鉴定和定量。在[R _˚F发现作为标准品以及叶提取物获得的槲皮素的值为 0.50。该方法在日内和日间精密度和准确度方面得到了验证。该方法的准确度由回收率研究确定，槲皮素的平均回收率为 98.04 ± 0.21%。冻干的香菜提取物样品含有 0.058 ± 0.02% 的槲皮素。HPTLC 方法导致开发了一种准确、精确、可重复和实用的方法来标准化C. sativum L.的水叶提取物。