Skip to main content
SpringerLink
Log in

Development of a validated high-performance thin-layer chromatography method for standardization of aqueous extract from the leaf of Coriandrum sativum L.

  • Original Research Paper
  • Published:
JPC – Journal of Planar Chromatography – Modern TLC Aims and scope Submit manuscript

Abstract

The purpose of the current investigation is to standardize the aqueous leaf extract of Coriandrum sativum L. with a high-performance thin-layer chromatography (HPTLC) method. The method involves the determination and validation of extract with quercetin as standard. Chromatography was performed on pre-coated silica gel G 60 F254 plate as the stationary phase with formic acid, ethyl acetate and toluene as the mobile phase in the ratio 1:4:5 (V/V). Identification and quantification were conducted optical densitometrically at 254 nm. The RF value of quercetin obtained for the standard as well as the leaf extract was found to be 0.50. The method was validated for intra-day and inter-day precision and accuracy. The accuracy of the method was established by a recovery study, with 98.04 ± 0.21% average recovery of quercetin. The lyophilized coriander extract samples contained 0.058 ± 0.02% quercetin. The HPTLC method resulted in the development of an accurate, precise, reproducible and pragmatic approach to standardize the aqueous leaf extract of C. sativum L.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Ishikawa T, Kondo K, Kitajima J (2003) Water-soluble constituents of coriander. Chem Pharm Bull 51:32–39

    Article  CAS  Google Scholar 

  2. Al-Snafi AE (2016) A review on chemical constituents and pharmacological activities of Coriandrum sativum. IOSR J Pharm 6:17–42

    Google Scholar 

  3. Pathak NL, Kasture SB, Bhatt NM, Rathod JD (2011) Phytopharmacological properties of Coriandrum sativum as a potential medicinal tree: an overview. J App Pharm Sci 1:20–25

    Google Scholar 

  4. Rajeshwari U, Andallu B (2011) Medicinal benefits of coriander (Coriandrum sativum L.). Spatula DD 1:51–58

    Article  Google Scholar 

  5. Rathabai V, Kanimozhi D (2012) Evaluation of anti-microbial activity of Coriandrum sativum. Int J Sci Res 3:01–10

    Google Scholar 

  6. Emamghoreishi M, Heidari-Hamedani G (2006) Sedative-hypnotic activity of extracts and essential oil of coriander seeds. Iran J Med Sci 31:22–27

    Google Scholar 

  7. Aissaoui A, Zizi S, Israili ZH, Lyoussi B (2011) Hypoglycemic and Hypolipidemic effects of Coriandrum sativum L. in Merionesshawi rats. J Ethnopharmacol 137:652–661

    Article  Google Scholar 

  8. Asgarpanah J, Kazemivash N (2012) Phytochemistry, pharmacology and medicinal properties of Coriandrum sativum L. Afr J Pharm Pharmaco 6:2340–2345

    CAS  Google Scholar 

  9. Liu QF, Jeong H, Lee JH, Hong YK, Oh Y, Kim YM, Suh YS, Bang S, Yun HS, K. Lee K, Cho SM, Lee SB, Jeon S, Chin YW, Koo BS, Cho KS, (2016) Coriandrum sativum Suppresses Aβ42-Induced ROS Increases, Glial Cell Proliferation, and ERK Activation. Am J Chin Med 44:1325–1347

    Article  CAS  Google Scholar 

  10. Hashim M, Lincy S, Remya V, Teena M, Anila L (2005) Effect of polyphenolic compounds from Coriandrum sativum on H2O2-induced oxidative stress in human lymphocytes. Food Chem 92:653–660

    Article  CAS  Google Scholar 

  11. Nambiar VS, Daniel M, Guin P (2010) Characterization of polyphenols from coriander leaf (Coriandrum sativum), red amaranthus (A. paniculatus) and green amaranthus (A. frumentaceus) using paper chromatography and their health implications. J Herb Med Toxicol 4:173–177

    Google Scholar 

  12. Saranya S, Eswari A, Gayathri E, Eswari S, Vijayarani K (2017) Green synthesis of metallic nanoparticles using aqueous plant extract and their antibacterial activity. Int J Curr Microbiol Appl Sci 6:1834–1845

    Article  CAS  Google Scholar 

  13. Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13:2638–2650

    Article  CAS  Google Scholar 

  14. Bhat S, Kaushal P, Kaur M, Sharma HK (2014) Coriander (Coriandrum sativum L.): processing, nutritional and functional aspects. Afr J Plant Sci 8:25–33

    Article  Google Scholar 

  15. Mukherjee D, Kumar NS, Khatua T, Mukherjee PK (2010) Rapid validated HPTLC method for estimation of betulinic acid in Nelumbo nucifera (Nymphaeaceae) rhizome extract. Phytochem Anal 21:556–560

    Article  CAS  Google Scholar 

  16. Ilbeigi V, Tabrizchi M (2015) Thin layer chromatography-ion mobility spectrometry (TLC-IMS). Anal Chem 87:464–469

    Article  CAS  Google Scholar 

  17. Wang T, Jin X, Chen Z, Megharaj M, Naidu R (2014) Green synthesis of Fe nano particles using eucalyptus leaf extracts for treatment of eutrophic wastewater. Sci Total Environ 466–467:210–213

    Article  Google Scholar 

  18. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199–1200

    Article  CAS  Google Scholar 

  19. Robak J, Gryglewski RJ (1988) Flavonoids are scavengers of super-oxides anions. Biochem Pharmacol 37:837–841

    Article  CAS  Google Scholar 

  20. Pick E, Mizel D (1981) Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. J Immunol Methods 46:211–226

    Article  CAS  Google Scholar 

  21. Aruoma OI (1999) Free radicals, antioxidants and international nutrition. Asia Pac J Clin Nut 8:53-e63

    Article  CAS  Google Scholar 

  22. Sethi PD (1996) HPTLC, 1st edn. CBS publishers and distributors, New Delhi, p 39

    Google Scholar 

  23. ICH Harmonised Tripartite Guideline (2005) Validation of analytical procedures: text and Methodology, Q2 (R1) Geneva

  24. Ferenczi-Fodor K, Renger B, Vegh Z (2010) The frustrated reviewer—recurrent failures in manuscripts describing validation of quantitative TLC/HPTLC procedures for analysis of pharmaceuticals. J Planar Chromatogr 23:173–179

    Article  CAS  Google Scholar 

  25. Ferenczi-Fodor K, Nagy-Turák A (1995) Validation and monitoring of quantitative thin layer chromatographic purity tests for bulk drug substances. J Planar Chromatogr 8:349–356

    CAS  Google Scholar 

Download references

Acknowledgements

We are grateful to the Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, Punjab, India.

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, 147002, India

    Kuldeep Singh, Dimple Sethi Chopra, Dhandeep Singh & Nirmal Singh

Authors
  1. Kuldeep Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dimple Sethi Chopra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dhandeep Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nirmal Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dimple Sethi Chopra.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Conflict of interest

The authors have no known conflicts of interest associated with this article.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Singh, K., Chopra, D.S., Singh, D. et al. Development of a validated high-performance thin-layer chromatography method for standardization of aqueous extract from the leaf of Coriandrum sativum L.. JPC-J Planar Chromat 34, 337–343 (2021). https://doi.org/10.1007/s00764-021-00121-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00764-021-00121-9

Keywords

Search

Navigation