Abstract

We performed a chromatographic evaluation of the recoveries of native forms of the main isoflavonoids from different morphological parts of red clover (Trifolium pratense L.) into water−alcoholic extracts using various extraction methods. Water−alcoholic extracts from red clover, obtained by maceration, microwave, ultrasonic, subcritical, and pharmacopoeial methods, were analyzed. Formononetin (0.260 mg/g) and genistein (0.051 mg/g) were maximally extracted from inflorescences of red clover by maceration; bioch-anin A was extracted by subcritical extraction (0.340 mg/g), and daidzein was extracted by ultrasonic method (0.034 mg/g). The conditions for the HPLC determination of daidzein, genistein, formononetin, and biochanin A in water−alcoholic extracts from red clover were optimized. The concentrations of isoflavonoids in various morphological parts of red clover were determined. The maximum total amount of isoflavonoids (0.190 mg/g) was observed in the water–ethanol extract from inflorescences.

中文翻译：

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使用不同方法提取豆科植物红三叶草（Trifolium pratense L.）中异黄酮的浓度评估

摘要

我们对来自红三叶草 ( Trifolium pratense)不同形态部分的主要异黄酮的天然形式的回收率进行了色谱评估L.) 使用各种提取方法制成水醇提取物。分析了通过浸渍、微波、超声波、亚临界和药典方法从红三叶草中提取的水醇提取物。浸渍法从红三叶草的花序中最大程度地提取了芒柄花素（0.260 mg/g）和染料木黄酮（0.051 mg/g）；亚临界萃取法提取生物茶素A（0.340 mg/g），超声法提取黄豆苷元（0.034 mg/g）。优化了 HPLC 测定红三叶草水-酒精提取物中黄豆苷元、染料木素、芒柄花素和生物链素 A 的条件。测定了红三叶草不同形态部位的异黄酮浓度。在花序的水-乙醇提取物中观察到异黄酮的最大总量 (0.190 mg/g)。