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Assessment of the Concentrations of Isoflavonoids in Red Clover (Trifolium pratense L.) of the Fabaceae Family Using Extraction by Different Methods

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Abstract

We performed a chromatographic evaluation of the recoveries of native forms of the main isoflavonoids from different morphological parts of red clover (Trifolium pratense L.) into water−alcoholic extracts using various extraction methods. Water−alcoholic extracts from red clover, obtained by maceration, microwave, ultrasonic, subcritical, and pharmacopoeial methods, were analyzed. Formononetin (0.260 mg/g) and genistein (0.051 mg/g) were maximally extracted from inflorescences of red clover by maceration; bioch-anin A was extracted by subcritical extraction (0.340 mg/g), and daidzein was extracted by ultrasonic method (0.034 mg/g). The conditions for the HPLC determination of daidzein, genistein, formononetin, and biochanin A in water−alcoholic extracts from red clover were optimized. The concentrations of isoflavonoids in various morphological parts of red clover were determined. The maximum total amount of isoflavonoids (0.190 mg/g) was observed in the water–ethanol extract from inflorescences.

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ACKNOWLEDGMENTS

The work is performed using scientific equipment from the Center for Collective Use “Ecological Analytical Center” of the Kuban State University.

Funding

This work was supported by the Russian Foundation for Basic Research (project no. FZEN-2020-0022).

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Authors and Affiliations

  1. Kuban State University, 350040, Krasnodar, Russia

    Z. A. Temerdashev, T. K. Chubukina, E. A. Vinitskaya, M. V. Nagalevskii & N. V. Kiseleva

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  1. Z. A. Temerdashev
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  2. T. K. Chubukina
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  3. E. A. Vinitskaya
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  4. M. V. Nagalevskii
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  5. N. V. Kiseleva
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Correspondence to Z. A. Temerdashev.

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Translated by O. Zhukova

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Temerdashev, Z.A., Chubukina, T.K., Vinitskaya, E.A. et al. Assessment of the Concentrations of Isoflavonoids in Red Clover (Trifolium pratense L.) of the Fabaceae Family Using Extraction by Different Methods. J Anal Chem 76, 1071–1082 (2021). https://doi.org/10.1134/S1061934821090112

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  • DOI: https://doi.org/10.1134/S1061934821090112

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