A New Minimally Invasive Method of Transverse Aortic Constriction in Mice,Journal of Cardiovascular Translational Research

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A New Minimally Invasive Method of Transverse Aortic Constriction in Mice
Journal of Cardiovascular Translational Research ( IF 2.4 ) Pub Date : 2021-09-08 , DOI: 10.1007/s12265-021-10170-4
Huayang Li _{1,

2} , Quan Liu _{1,

2} , Shunjun Wang ₁ , Lin Huang ₁ , Suiqing Huang ₁ , Yuan Yue ₁ , Kangni Feng ₁ , Zhongkai Wu _{1,

2}

Affiliation

Transverse aortic constriction (TAC) in mice is the most popular model to mimic pressure overload heart disease. In this study, we developed a convenient, quick, and less invasive new TAC mice model. Briefly, after anesthetization, endotracheal intubation was then performed, and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6–0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel. A blunted 27-gauge needle was placed parallel to the transverse aorta and then three knots were tied quickly. After ligation, the spacer was removed promptly and gently to achieve a constriction of 0.4 mm in diameter. Five weeks after TAC, cardiac hypertrophy, fibrosis, and left ventricular dysfunction were observed.

Graphical abstract

The mouse was anesthetized with pentobarbital (50 mg/kg) via intraperitoneal injection. Endotracheal intubation under direct vision was then performed and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6–0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel.

中文翻译：

一种新的小鼠横向主动脉缩窄微创方法

小鼠的横向主动脉缩窄 (TAC) 是模拟压力过载心脏病的最流行模型。在这项研究中，我们开发了一种方便、快速、侵入性较小的新型 TAC 小鼠模型。简而言之，在麻醉后，进行气管插管，并将气管插管连接到呼吸机。打开第二肋间，然后用自制的牵开器轻轻推开胸腺。在主动脉弓下制作了一个隧道，一段 6-0 单丝聚丙烯缝合线穿过一个特别改进的钝头 26 号注射器针头，穿过该隧道。将一根钝的 27 号针平行于横向主动脉放置，然后快速打结三个结。结扎后，迅速而轻柔地移除垫片，以实现直径 0.4 mm 的收缩。TAC 后 5 周，观察到心脏肥大、纤维化和左心室功能障碍。