Abstract
Transverse aortic constriction (TAC) in mice is the most popular model to mimic pressure overload heart disease. In this study, we developed a convenient, quick, and less invasive new TAC mice model. Briefly, after anesthetization, endotracheal intubation was then performed, and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6–0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel. A blunted 27-gauge needle was placed parallel to the transverse aorta and then three knots were tied quickly. After ligation, the spacer was removed promptly and gently to achieve a constriction of 0.4 mm in diameter. Five weeks after TAC, cardiac hypertrophy, fibrosis, and left ventricular dysfunction were observed.
Graphical abstract
The mouse was anesthetized with pentobarbital (50 mg/kg) via intraperitoneal injection. Endotracheal intubation under direct vision was then performed and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6–0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel.
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Abbreviations
- TAC:
-
Transverse aortic constriction
- LV:
-
Left ventricular
- BCA:
-
Brachiocephalic artery
- LCCA:
-
Left common carotid artery
- LVIDd:
-
LV end-diastolic internal diameter
- LVIDs:
-
LV end-systolic internal diameter
- IVSd:
-
Interventricular septum end-diastolic thickness
- IVSs:
-
Interventricular septum end-systolic thickness
- LVPWd:
-
LV end-diastolic posterior wall thickness
- LVPWs:
-
LV end-systolic posterior wall thickness
- LVEDV:
-
LV end-diastolic volume
- LVESV:
-
LV end-systolic volume
- LVEF:
-
LV ejection fraction
- LVFS:
-
LV fractional shortening
- HW:
-
Heart weight
- BW:
-
Body weight
- TL:
-
Tibia length
- H&E:
-
Hematoxylin-eosin
- MT:
-
Masson’s trichrome
- AAo:
-
Ascending aorta
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Funding
This study was funded by the National Natural Science Foundation of China (Grant No. 81770319).
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All authors contributed to the study conception and design. Huayang Li, Quan Liu, and Shunjun Wang carried out the experiments. Lin Huang, Yuan Yue, Suiqing Huang, and Kangni Feng helped with the material preparation, data collection, and analysis. The first draft of the manuscript was written by Huayang Li, and all authors commented on previous versions of the manuscript. Zhongkai Wu contributed substantially to the conception, design of the study, acquired funding, supervised the study, and edited the manuscript. All authors read and approved the final manuscript.
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No human studies were carried out by the authors for this article.
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All institutional and national guidelines for the care and use of laboratory animals were followed and approved by the Institutional Animal Use and Care Committee of Sun Yat-sen University (2019001114).
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The authors declare no competing interests.
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Huayang Li and Quan Liu contributed equally to this article.
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Li, H., Liu, Q., Wang, S. et al. A New Minimally Invasive Method of Transverse Aortic Constriction in Mice. J. of Cardiovasc. Trans. Res. 15, 635–643 (2022). https://doi.org/10.1007/s12265-021-10170-4
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DOI: https://doi.org/10.1007/s12265-021-10170-4