A novel, strictly anaerobic, gram-negative, segmented filamentous bacterium strain AGMB03513^T, was isolated from the faeces of a 5-month-old pig. Phylogenetic analysis based on the 16S rRNA gene indicated that the isolate was a member of the family Lachnospiraceae, and the closest strain was Anaerostipes butyraticus. Strain AGMB03513^T formed a lineage within the genus Anaerostipes and was closely related to A. butyraticus DSM 22094 ^T (= KCTC 15125 ^T, 95.8%), Anaerostipes hadrus DSM 3319 ^T (= KCTC 15606 ^T, 95.5%), Anaerostipes caccae DSM 14662 ^T (= KCTC 15019 ^T, 94.0%), and Anaerostipes rhamnosivorans DSM 26241 ^T (= KCTC 15316 ^T, 93.4%). Strain AGMB03513^T grew at temperatures between 30 and 45 °C, within a pH range of 7.0–9.0, and in medium containing up to 1.5% NaCl. Cells were found to utilise d-glucose, d-mannitol, d-lactose, d-saccharose, d-maltose, d-xylose, l-arabinose, d-mannose, and d-sorbitol, and acetate was identified as the major end product of metabolism. The major components of the cellular fatty acids were C_12:0, C_16:0, and C_18:0. In addition, the bacterium contained meso-diaminopimelic acid in the cell wall. According to the comparative analysis of the whole genome sequence, the DNA G + C content of strain AGMB03513 was 37.0 mol%. In addition, Average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridisation (dDDH) values were obtained in comparisons of strain AGMB03513^T with reference strains of species in the genus Anaerostipes. ANI values were found to be between 71.0 and 75.7%, AAI values between 66.6 and 73.2%, and dDDH values between 19.5 and 21.4%. All the data were below the threshold range for species determination. Based on phenotypic, phylogenetic, biochemical, chemotaxonomic, and genomic characteristics, we considered it reasonable to assign a novel species status to strain AGMB03513^T, for which we propose the name Anaerostipes faecalis sp. nov. The type strain is AGMB03513^T (= KCTC 25020 ^T = NBRC 114896 ^T).

从一头 5 个月大的猪的粪便中分离出一种新的、严格厌氧、革兰氏阴性、分段的丝状细菌菌株 AGMB03513 ^T。基于16S rRNA基因的系统发育分析表明，该分离物是毛螺科的一员，最接近的菌株是Anaerostipes butyraticus。应变AGMB03513 ^Ť形成属内谱系厌氧和密切相关A. butyraticus DSM 22094  ^Ť（= KCTC 15125  ^Ť，95.8％），厌氧hadrus DSM 3319  ^Ť（= KCTC 15606  ^Ť，95.5％），粪厌氧棒状菌DSM 14662  ^T（= KCTC 15019  ^T，94.0%）和鼠李糖厌氧菌DSM 26241  ^T（= KCTC 15316  ^T，93.4%）。菌株 AGMB03513 ^T在 30 到 45 °C 之间的温度、7.0–9.0 的 pH 范围内以及含有高达 1.5% NaCl 的培养基中生长。发现细胞利用d-葡萄糖、d-甘露糖醇、d-乳糖、d-蔗糖、d-麦芽糖、d-木糖、l-阿拉伯糖、d-甘露糖和d山梨糖醇和醋酸盐被确定为代谢的主要终产物。细胞脂肪酸的主要成分是C _12:0、C _16:0和C _18:0。此外，该细菌在细胞壁中含有内消旋二氨基庚二酸。根据全基因组序列对比分析，菌株AGMB03513的DNA G+C含量为37.0 mol%。此外，平均核苷酸同一性 (ANI)、平均氨基酸同一性 (AAI) 和数字 DNA-DNA 杂交 (dDDH) 值是在菌株 AGMB03513 ^T与厌氧菌属物种的参考菌株的比较中获得的。发现 ANI 值介于 71.0 和 75.7% 之间，AAI 值介于 66.6 和 73.2% 之间，dDDH 值介于 19.5 和 21.4% 之间。所有数据均低于物种确定的阈值范围。基于表型、系统发育、生化、化学分类学和基因组特征，我们认为为菌株 AGMB03513 ^T分配新物种状态是合理的，为此我们建议将其命名为Anerostipes faecalis sp。十一月 类型菌株是 AGMB03513 ^T (= KCTC 25020  ^T  = NBRC 114896  ^T )。