Abstract
A novel, strictly anaerobic, gram-negative, segmented filamentous bacterium strain AGMB03513T, was isolated from the faeces of a 5-month-old pig. Phylogenetic analysis based on the 16S rRNA gene indicated that the isolate was a member of the family Lachnospiraceae, and the closest strain was Anaerostipes butyraticus. Strain AGMB03513T formed a lineage within the genus Anaerostipes and was closely related to A. butyraticus DSM 22094 T (= KCTC 15125 T, 95.8%), Anaerostipes hadrus DSM 3319 T (= KCTC 15606 T, 95.5%), Anaerostipes caccae DSM 14662 T (= KCTC 15019 T, 94.0%), and Anaerostipes rhamnosivorans DSM 26241 T (= KCTC 15316 T, 93.4%). Strain AGMB03513T grew at temperatures between 30 and 45 °C, within a pH range of 7.0–9.0, and in medium containing up to 1.5% NaCl. Cells were found to utilise d-glucose, d-mannitol, d-lactose, d-saccharose, d-maltose, d-xylose, l-arabinose, d-mannose, and d-sorbitol, and acetate was identified as the major end product of metabolism. The major components of the cellular fatty acids were C12:0, C16:0, and C18:0. In addition, the bacterium contained meso-diaminopimelic acid in the cell wall. According to the comparative analysis of the whole genome sequence, the DNA G + C content of strain AGMB03513 was 37.0 mol%. In addition, Average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridisation (dDDH) values were obtained in comparisons of strain AGMB03513T with reference strains of species in the genus Anaerostipes. ANI values were found to be between 71.0 and 75.7%, AAI values between 66.6 and 73.2%, and dDDH values between 19.5 and 21.4%. All the data were below the threshold range for species determination. Based on phenotypic, phylogenetic, biochemical, chemotaxonomic, and genomic characteristics, we considered it reasonable to assign a novel species status to strain AGMB03513T, for which we propose the name Anaerostipes faecalis sp. nov. The type strain is AGMB03513T (= KCTC 25020 T = NBRC 114896 T).
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Abbreviations
- AGMB:
-
Animal gut microbiome bank
- KCTC:
-
Korean collection for type cultures
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Acknowledgements
We thank Professor Bernhard Schink for his advice on naming the novel strain.
Funding
This work was supported by the Bio & Medical Technology Development Program (Project Nos. NRF-2019M3A9F3065226 and NRF-2021M3H9A1030164) of the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT (MSIT) of the South Korea and by the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (KGM5232113). S.-H.P. was supported by the Technology Innovation Program (20009412, Discovery and fermentation optimisation of uncultured bacteria from the gut microbiome based on genomic big data), funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea). J.-S. L. was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2016M3A9F3946674).
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SHP, YH, SHC, JYC designed and coordinated this study; SHC and JYC performed the experiments and drafted the manuscript; JEP and JSK helped with the experiments and interpreted the results; SWK, JL, MKL, JSL, and JHL collected the samples and helped in the experiments; SHP, HJ, TH, HBK, JHL, and JKK revised the manuscript.
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The experimental protocols for this study were reviewed and approved by the Institutional Animal Care and Use Committee of the National Institute of Animal Science (NIAS-2019-1731).
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Choi, SH., Choi, J.Y., Park, JE. et al. Description of Anaerostipes faecalis sp. nov., a new segmented filamentous bacterium isolated from swine faeces. Antonie van Leeuwenhoek 114, 1867–1875 (2021). https://doi.org/10.1007/s10482-021-01646-z
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DOI: https://doi.org/10.1007/s10482-021-01646-z