Purpose

Influenza is one of the most important agents of pandemic outbreak causing substantial morbidity and mortality. Vaccination strategies of influenza must be adapted annually due to constant antigenic changes in various strains. Therefore, the present study was conducted to evaluate protective immunity of the conserved influenza proteins.

Methods

For this purpose, three tandem repeats of M2e (3M2e) and NP were separately expressed in E. coli and were purified using column chromatography. Female Balb/c mice were injected intradermally with a combination of the purified 3M2e and NP alone or formulated with Alum (AlOH₃) adjuvant in three doses. The mice were challenged by intranasal administration of H1N1 (A/PR/8/34) 2 weeks after the last vaccination.

Results

The results demonstrated that recombinant NP and M2e proteins are immunogenic and could efficiently elicit immune responses in mice compared to non-immunized mice. The combination of 3M2e and NP supplemented with Alum stimulated both NP and M2e-specific antibodies, which were higher than those stimulated by each single antigen plus Alum. In addition, the secretion of IFN-γ and IL-4 as well as the induction of lymphocyte proliferation in mice received the mixture of these proteins with Alum was considerably higher than other groups. Moreover, the highest survival rate (86%) with the least body weight change was observed in the mice immunized with 3M2e and NP supplemented with Alum followed by the mice received NP supplemented with Alum (71%).

Conclusion

Accordingly, this regimen can be considered as an attractive candidate for global vaccination against influenza.

中文翻译：

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用明矾保护的 BALB/c 小鼠配制的保守重组蛋白（NP 和 3M2e）的组合对抗甲型流感/PR8/H1N1 病毒攻击

目的

流感是大流行爆发的最重要因素之一，导致大量发病率和死亡率。由于各种毒株的抗原不断变化，必须每年调整流感疫苗接种策略。因此，本研究旨在评估保守流感蛋白的保护性免疫。

方法

为此，M2e (3M2e) 和 NP 的三个串联重复在大肠杆菌中分别表达，并使用柱色谱法纯化。雌性 Balb/c 小鼠皮内注射纯化的 3M2e 和 NP 的组合，或与明矾 (AlOH ₃ ) 佐剂一起配制三个剂量。在最后一次疫苗接种后 2 周，通过鼻内施用 H1N1 (A/PR/8/34) 对小鼠进行攻击。

结果

结果表明，与未免疫小鼠相比，重组 NP 和 M2e 蛋白具有免疫原性，可以有效地引发小鼠的免疫反应。补充明矾的 3M2e 和 NP 的组合刺激了 NP 和 M2e 特异性抗体，其高于每种单一抗原加明矾刺激的抗体。此外，在接受这些蛋白质与明矾混合物的小鼠中，IFN-γ和IL-4的分泌以及对淋巴细胞增殖的诱导显着高于其他组。此外，在用 3M2e 和添加明矾的 NP 免疫的小鼠中观察到最高的存活率（86%）和最小的体重变化，其次是接受添加了明矾的 NP 的小鼠（71%）。

结论

因此，该方案可被视为全球流感疫苗接种的有吸引力的候选方案。