By applying the mixed-ligand approach, two coordination polymers (CPs) based on Cu(II) of {[Cu(3-bpdb)(fum)(H₂O)₂]}_n (1, 3-bpdb = 1,4-bis(3-pyridyl)-2,3-diaza-1,3-butadiene and fum = fumarate) and {[Cu₄(4-bpdb)₂(suc)₃(OH)₂]·5(H₂O)}_n (2, 4-bpdb = 1,4-bis(4-pyridyl)-2,3-diaza-1,3-butadiene and suc = succinate) were triumphantly synthesized via a slow volatilization method. The magnetic properties for complexes 1 and 2 have been studied, which reveal their antiferromagnet interactions among the metal ions. Their protective activity on the restless leg syndrome was evaluated. First of all, the tyrosine hydroxylase relative expression in dopaminergic neuron of anterior horn of spinal cord was detected through the real time reverse transcription-polymerase chain reaction (RT-PCR) method. In addition to this, the inflammatory response in the anterior horn of spinal cord was also assessed by determining the inflammatory cytokines levels by enzyme linked immunosorbent assay (ELISA) kit.

通过应用混合配体方法，两种基于 Cu(II) 的配位聚合物 (CP) {[Cu(3-bpdb)(fum)(H ₂ O) ₂ ]} _n ( 1 , 3-bpdb = 1, 4-bis(3-pyridyl)-2,3-diaza-1,3-butadiene and fum = fumarate) 和 {[Cu ₄ (4-bpdb) ₂ ( suc ) ₃ (OH) ₂ ]·5(H ₂ O)} _n ( 2 , 4-bpdb = 1,4-bis(4-pyridyl)-2,3-diaza-1,3-butadiene and suc = succinate) 通过慢挥发法成功合成。配合物1和2的磁性已经研究过，这揭示了它们在金属离子之间的反铁磁相互作用。评估了它们对不宁腿综合征的保护活性。首先，通过实时逆转录-聚合酶链反应（RT-PCR）方法检测脊髓前角多巴胺能神经元中酪氨酸羟化酶的相对表达量。除此之外，还通过酶联免疫吸附测定（ELISA）试剂盒测定炎性细胞因子水平来评估脊髓前角的炎症反应。