An endophytic actinomycete, designated strain RL4-1S^T, was isolated from surface-sterilized leaves of rice plant (Oryza sativa L.) collected from Buri Rum province, Thailand. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequences showed that the strain RL4-1S^T belongs to the genus Microbispora and is most closely related to Microbispora rosea subsp. rosea ATCC 12950^T (98.5%). The strain forms pairs of spores on short sporophores borne on the aerial mycelium. Polar lipid profile of strain RL4-1S^T is diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, ninhydrin-positive glycophospholipid, two unidentified phospholipids, an unidentified aminolipid, and an unidentified glycolipid. MK-9(H₄), MK-9(H₂), and MK-9 are major menaquinones of this organism. The predominant cellular fatty acids are iso-C_16:0, C_17:0 and C_16:0. Strain RL4-1S^T contains meso-diaminopimelic acid, glucose, madurose and ribose in whole-cell hydrolysates. The draft genome of strain RL4-1S^T consists of 7.46 Mbp and has a G + C content 71.2 mol%. Digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain RL4-1S^T with Microbispora rosea subsp. rosea ATCC 12950^T are 26.0% and 80.7%, respectively. Based on data of genotypic, phenotypic, phylogenetic and chemotaxonomic analysis, strain RL4-1S^T represents a novel species of the genus Microbispora, for which the name Microbispora oryzae sp. nov. is proposed. The type strain is RL4-1S^T (= TBRC 14817^T = NBRC 115115^T).

一种内生放线菌，命名为 RL4-1S ^T菌株，是从从泰国武里南省收集的水稻植物 ( Oryza sativa L.)的表面灭菌叶子中分离出来的。它的分类地位是使用多相方法确定的。基于16S rRNA 基因序列的系统发育分析表明菌株RL4-1S T^属于微双孢菌属，与微双孢菌rosea亚种关系最密切。蔷薇ATCC 12950 ^T (98.5%)。该菌株在气生菌丝体上的短子实体上形成成对的孢子。菌株 RL4-1S ^{T的极性脂质谱}是二磷脂酰甘油、羟基磷脂酰乙醇胺、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、茚三酮阳性糖磷脂、两种不明磷脂、一种不明氨基脂和一种不明糖脂。MK-9(H ₄ )、MK-9(H ₂ ) 和 MK-9 是该生物体的主要甲萘醌。主要的细胞脂肪酸是异-C _16:0、C _17:0和 C _16:0。菌株 RL4-1S ^T在全细胞水解产物中含有内消旋-二氨基庚二酸、葡萄糖、马杜糖和核糖。菌株 RL4-1S ^{T的基因组草图}由 7.46 Mbp 组成，G + C 含量为 71.2 mol%。^{菌株 RL4-1S T}与Microbispora rosea亚种的基因组序列之间的数字 DNA-DNA 杂交和平均核苷酸同一性值。rosea ATCC 12950 ^T分别为 26.0% 和 80.7%。基于基因型、表型、系统发育和化学分类学分析的数据，菌株 RL4-1S ^T代表小双孢菌属的一个新物种，其名称为Microbispora oryzae sp. 十一月 被提议。类型菌株是 RL4-1S ^T（= TBRC 14817 ^T  = NBRC 115115 ^T）。