An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110^T, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110^T was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541^T (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491^T (99.1%), Streptomyces luteoverticillatus NBRC 3840^T (99.1%), Streptomyces coerulescens NBRC 12758^T (99.1%), and Streptomyces iranensis HM 35^T (99.0%). Strain CA3R110^T contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H₆), and MK-9(H₈) as major menaquinone, iso-C_16:0, iso-C_15:0, C_16:0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110^T could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110^T represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110^T (= TBRC 11245^T = NBRC 114296^T).

从泰国南邦省收集的阿拉比卡咖啡表面灭菌根中分离出一种需氧、非运动性、革兰氏染色阳性放线菌，命名为 CA3R110 ^T菌株。16S rRNA基因序列分析表明菌株CA3R110 ^Ť是属的成员链霉菌和显示最接近的相似性链霉菌buecherae AC541 ^Ť（99.2％），其次是链霉菌rapamycinicus NRRL B-5491 ^Ť（99.1％），链霉菌属luteoverticillatus NBRC 3840 ^T (99.1%)，蓝斑链霉菌NBRC 12758 ^T(99.1%) 和伊朗链霉菌HM 35 ^T (99.0%)。菌株 CA3R110 ^T在细胞肽聚糖中含有LL-二氨基庚二酸、MK-9(H ₆ ) 和 MK-9(H ₈ ) 作为主要甲基萘醌、iso -C _16:0、 iso -C _15:0、 C _16:0作为主要的脂肪酸。在细胞中检测到二磷脂酰甘油、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷。化学分类特征具有链霉菌属的典型特性. 低数字 DNA-DNA 杂交 (<55.7%) 和平均核苷酸同一性爆炸 (ANIb) (<92.2%) 值表明菌株 CA3R110 ^T可以与任何已知的链霉菌属物种区分开来。由于表型和基因型数据的差异，菌株 CA3R110 ^T代表了Streptomyces属的一个新物种，其名称为Streptomyces endocoffeicus sp。十一月 被提议。类型菌株是 CA3R110 ^T (= TBRC 11245 ^T  = NBRC 114296 ^T )。