Probiotics are microbes having tremendous potential to prevent gastrointestinal disorders. In current investigation, immunomodulatory action of probiotic Lacticaseibacillus rhamnosus MTCC-5897 was studied during exclusion, competition and displacement of Escherichia coli on intestinal epithelial (Caco-2) cells. The incubation of intestinal cells with Escherichia coli, enhanced downstream signalling and activated nuclear factor kappa B (NF-κB). This significantly increased (p < 0.01) the pro-inflammatory cytokines (IL-8, TNF-α, IFN-ϒ) expression. While, incubation of epithelial cells with Lacticaseibacillus rhamnosus during exclusion and competition with Escherichia coli, counteracted these enhanced expressions. The immunomodulatory feature of Lacticaseibacillus rhamnosus was also highlighted with increased (p < 0.05) transcription of toll-like receptor-2 (TLR-2) and single Ig IL-1-related receptor (SIGIRR) along with diminished expression of TLR-4. Likewise, attenuation (p < 0.05) of E. coli-mediated enhanced nuclear translocation of NF-κB p-65 subunit by Lacticaseibacillus rhamnosus during exclusion was confirmed with western blotting. Thus, present finding establishes the prophylactic potential of Lacticaseibacillus rhamnosus against exclusion of Escherichia coli in intestinal cells.

益生菌是具有预防胃肠道疾病的巨大潜力的微生物。在目前的调查中，益生菌鼠李糖乳杆菌 MTCC-5897 在大肠杆菌对肠上皮 (Caco-2) 细胞的排斥、竞争和置换过程中的免疫调节作用进行了研究 。肠道细胞与大肠杆菌一起孵育，增强了下游信号传导并激活了核因子 kappa B (NF-κB)。这显着增加了（p  < 0.01）促炎细胞因子（IL-8、TNF-α、IFN-ϒ）的表达。而上皮细胞与鼠李糖乳杆菌在排斥和与大肠杆菌竞争的过程中孵育，抵消了这些增强的表达。鼠李糖乳杆菌的免疫调节特征也随着 toll 样受体 2 (TLR-2) 和单个 Ig IL-1 相关受体 (SIGIRR) 的转录增加 ( p < 0.05) 以及 TLR-4 表达的减少而突出。同样，通过蛋白质印迹证实了大肠杆菌介导的鼠李糖乳杆菌在排除过程中增强的 NF-κB p-65 亚基核转位的减弱 ( p  < 0.05) 。因此，目前的发现确定了鼠李糖乳杆菌 对肠细胞中大肠杆菌的排除的预防潜力。