Abstract
Probiotics are microbes having tremendous potential to prevent gastrointestinal disorders. In current investigation, immunomodulatory action of probiotic Lacticaseibacillus rhamnosus MTCC-5897 was studied during exclusion, competition and displacement of Escherichia coli on intestinal epithelial (Caco-2) cells. The incubation of intestinal cells with Escherichia coli, enhanced downstream signalling and activated nuclear factor kappa B (NF-κB). This significantly increased (p < 0.01) the pro-inflammatory cytokines (IL-8, TNF-α, IFN-ϒ) expression. While, incubation of epithelial cells with Lacticaseibacillus rhamnosus during exclusion and competition with Escherichia coli, counteracted these enhanced expressions. The immunomodulatory feature of Lacticaseibacillus rhamnosus was also highlighted with increased (p < 0.05) transcription of toll-like receptor-2 (TLR-2) and single Ig IL-1-related receptor (SIGIRR) along with diminished expression of TLR-4. Likewise, attenuation (p < 0.05) of E. coli-mediated enhanced nuclear translocation of NF-κB p-65 subunit by Lacticaseibacillus rhamnosus during exclusion was confirmed with western blotting. Thus, present finding establishes the prophylactic potential of Lacticaseibacillus rhamnosus against exclusion of Escherichia coli in intestinal cells.
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Acknowledgements
The authors acknowledge ICAR-National Dairy Research Institute (NDRI) Karnal, for providing the laboratory facilities for execution of this work. We are also thankful to Department of Biotechnology, Ministry of Science and Technology, New Delhi for providing required funds.
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The authors are highly indebted to the Department of Biotechnology, Ministry of Science and Technology, New Delhi for providing necessary funds. (BT/PR15109/PFN/20/1174/2015) required for the present work. The funding agency has no role in the design, analysis or writing of this article.
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TG, RK: Conceptualization; TG, HK: methodology; TG, RK, SK: formal analysis; TG: investigation; TG: writing—original draft; HK, SK: writing—review and editing; TG, HK: software; TG, RK: validation; RK: funding acquisition; RK: resources; RK: supervision.
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Gupta, T., Kaur, H., Kapila, S. et al. Potential probiotic Lacticaseibacillus rhamnosus MTCC-5897 attenuates Escherichia coli induced inflammatory response in intestinal cells. Arch Microbiol 203, 5703–5713 (2021). https://doi.org/10.1007/s00203-021-02541-x
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DOI: https://doi.org/10.1007/s00203-021-02541-x