Head smut (HS) is one of the most devastating diseases of maize in spring production regions in China. Quantitative trait loci (QTL) for HS resistance were identified in this study to provide theoretical and applied tools for breeding HS resistance in maize. QTL associated with HS resistance were identified in a F_2:3 population derived from a T32 (highly resistant genotype) × HC (highly susceptible genotype) cross. Analysis in each of three environments and a collective analysis across all three environments were used to identify QTL in the F_2:3 population. A significant difference in HS resistance was found between the inbred lines, ‘T32’ and ‘HC’. Large genetic variation and transgressive segregation in the F_2:3 population were observed between the three different sites, Guian (GA), Huaxi (HX), and Pingba (PB). Two stable and novel QTL for resistance to HS were detected in the different environments that were located within the bnlg1014 to umc2224 (qHS1) interval on chromosome 1 and in the umc1006 to umc1857 (qHS6) interval on chromosome 6. Both QTL can be used for further fine mapping, marker-assisted selection breeding, and theoretical studies on HS resistance in maize.

黑穗病（HS）是我国春季产区玉米的主要病害之一。本研究鉴定了HS抗性的数量性状基因座（QTL），为玉米HS抗性育种提供理论和应用工具。在源自 T32（高抗基因型）×HC（高敏感基因型）杂交的 F _2:3群体中鉴定出与 HS 抗性相关的 QTL 。在三个环境中的每一个环境中的分析和在所有三个环境中的集体分析用于鉴定 F _2:3群体中的 QTL 。在自交系“T32”和“HC”之间发现了 HS 抗性的显着差异。F _2:3中的大遗传变异和海侵分离在贵安 (GA)、华西 (HX) 和平坝 (PB) 三个不同地点之间观察到种群。在分别位于bnlg1014内umc2224（即不同的环境中检测到两种稳定和用于向HS抗性QTL新颖qHS1）间隔1号染色体上，并在到umc1006 umc1857（qHS6）间隔上6.染色体两种QTL可用于进一步精细定位、标记辅助选择育种和玉米抗HS的理论研究。