Two new trihydroxy derivative of Δ^8(14),15-isopimarane diterpenoids, shanpanootols G (1) and H (2), along with three known analogues were isolated from the ethyl acetate-soluble extract of Kaempferia pulchra rhizomes collected in Shan State of Myanmar. The structures of these compounds including their absolute configurations were elucidated by the combination of one dimensional (1D) and 2D-NMR spectroscopic methods, high resolution mass spectrometric technique, and the experimental and the calculated electronic circular dichroism (ECD) data. The isopimarane diterpenoids (1–5) were tested for their Viral protein R (Vpr) inhibitory activities against TREx-HeLa-Vpr cells. Shanpanootol H (2) and (1R,2S,5S,9R,10S,13R)-1,2-dihydroxypimara-8(14),15-dien-7-one (4) exhibited anti-Vpr activities at the 5 µM treated dose.

从掸邦采集的山柰根茎的乙酸乙酯可溶性提取物中分离出两种新的 Δ ^8(14),15-异海松烷二萜三羟基衍生物, shanpanootols G ( 1 ) 和 H ( 2 ) 以及三个已知的类似物。缅甸。这些化合物的结构包括它们的绝对构型通过一维 (1D) 和 2D-​​NMR 光谱方法、高分辨率质谱技术以及实验和计算电子圆二色性 (ECD) 数据的组合阐明。异海松烷二萜 ( 1 – 5) 测试了它们对 TREx-HeLa-Vpr 细胞的病毒蛋白 R (Vpr) 抑制活性。Shanpanotol H ( 2 ) 和 (1 R ,2 S ,5 S ,9 R ,10 S ,13 R )-1,2-dihydroxypimara-8(14),15-dien-7-one ( 4 ) 表现出抗- 5 µM 处理剂量下的 Vpr 活性。