Owing to the prominent capabilities of bioconversion and biosynthesis, A. terreus has become attractive in biotechnical and pharmaceutical industry. In this work, an Aspergillus strain with potential antibacterial activities, was isolated from sponge in South China Sea. Based on the morphological and phylogenetic analysis, the strain was identified as A. terreus B12. Via the Illumina MiSeq sequencing platform, the complete genome was obtained, showing a genetic richness of biosynthetic gene clusters (BGCs), which might underpin the metabolic plasticity and adaptive resilience for the strain. Genome mining identified 67 BGCs, among which, 6 gene clusters could allocate to known BGCs (100% identity), corresponding to diverse metabolites like clavaric acid, dihydroisoflavipucine/isoflavipucine, dimethylcoprogen, alternariol, aspterric acid, and pyranonigrin E. Moreover, a range of compounds was isolated from B12 fermentation, e.g., terrein, butyrolactone I, terretonin A&E, acoapetaline B, and epi-aszonalenins A. Of note, acoapetaline B and epi-aszonalenins A, which had been respectively reported in plants and A. novofumigatus but with scarce information, was unexpectedly obtained from this species for the first time. The genomic and metabolic heterogeneity observed in strain B12, should be at least partially attributed to the genetic variability and biochemical diversity of A. terreus, which could be an interesting issue open to future efforts.

中文翻译：

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海洋来源的土曲霉 B12 次级代谢产物的基因组挖掘。

由于生物转化和生物合成的突出能力，A. terreus 在生物技术和制药工业中变得很有吸引力。在这项工作中，从南海海绵中分离出一株具有潜在抗菌活性的曲霉属菌株。基于形态学和系统发育分析，该菌株被鉴定为A. terreus B12。通过 Illumina MiSeq 测序平台，获得了完整的基因组，显示出生物合成基因簇 (BGC) 的遗传丰富性，这可能支持该菌株的代谢可塑性和适应性恢复力。基因组挖掘确定了 67 个 BGCs，其中 6 个基因簇可以分配给已知的 BGCs（100% 同一性），对应于多种代谢物，如克拉维酸、二氢异黄酮碱/异黄酮碱、二甲基肝原、交替醇、天冬氨酸、此外，从 B12 发酵中分离出一系列化合物，例如，terrein、丁内酯 I、terretonin A&E、acoapetaline B 和 epi-aszonalenins A。值得注意的是，acoapetaline B 和 epi-aszonalenins A，它们分别是在植物和 A. novofumigatus 中报道但信息稀缺，是首次意外地从该物种中获得。在菌株 B12 中观察到的基因组和代谢异质性，应该至少部分归因于 A. terreus 的遗传变异性和生化多样性，这可能是一个有待于未来努力的有趣问题。分别在植物和 A. novofumigatus 中报道但信息稀少的，意外地首次从该物种中获得。在菌株 B12 中观察到的基因组和代谢异质性，应该至少部分归因于 A. terreus 的遗传变异性和生化多样性，这可能是一个有待于未来努力的有趣问题。分别在植物和 A. novofumigatus 中报道但信息稀少的，意外地首次从该物种中获得。在菌株 B12 中观察到的基因组和代谢异质性，应该至少部分归因于 A. terreus 的遗传变异性和生化多样性，这可能是一个有待于未来努力的有趣问题。