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Genome mining of secondary metabolites from a marine-derived Aspergillus terreus B12

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Abstract

Owing to the prominent capabilities of bioconversion and biosynthesis, A. terreus has become attractive in biotechnical and pharmaceutical industry. In this work, an Aspergillus strain with potential antibacterial activities, was isolated from sponge in South China Sea. Based on the morphological and phylogenetic analysis, the strain was identified as A. terreus B12. Via the Illumina MiSeq sequencing platform, the complete genome was obtained, showing a genetic richness of biosynthetic gene clusters (BGCs), which might underpin the metabolic plasticity and adaptive resilience for the strain. Genome mining identified 67 BGCs, among which, 6 gene clusters could allocate to known BGCs (100% identity), corresponding to diverse metabolites like clavaric acid, dihydroisoflavipucine/isoflavipucine, dimethylcoprogen, alternariol, aspterric acid, and pyranonigrin E. Moreover, a range of compounds was isolated from B12 fermentation, e.g., terrein, butyrolactone I, terretonin A&E, acoapetaline B, and epi-aszonalenins A. Of note, acoapetaline B and epi-aszonalenins A, which had been respectively reported in plants and A. novofumigatus but with scarce information, was unexpectedly obtained from this species for the first time. The genomic and metabolic heterogeneity observed in strain B12, should be at least partially attributed to the genetic variability and biochemical diversity of A. terreus, which could be an interesting issue open to future efforts.

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Abbreviations

BGCs:

Biosynthetic gene clusters

SMs:

Secondary metabolites

PDB:

Potato Dextrose Broth

MH:

Mueller–Hinton

MEA:

Malt Extract Agar

MeOH:

Methanol; LPCB, lactophenol cotton blue

ITS:

Internal transcribed spacer

ML:

Maximum-likelihood

GO:

Gene Ontology

KEGG:

Kyoto Encyclopedia of Genes and Genomes

CAZy:

Carbohydrate-Active enZYmes

COG:

Clusters of Orthologous Groups

NRPS:

Nonribosomal peptide synthase

PKS:

Polyketide synthase

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Acknowledgements

This work was supported by funds from the National Key R&D Program of China (2018YFC0311000), the Priority Academic Program Development of the Jiangsu Higher Education Institutions (PAPD).

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Correspondence to Ying Wang.

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The authors declare no conflict of interest.

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Communicated by Erko Stackebrandt.

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Du, X., Li, H., Qi, J. et al. Genome mining of secondary metabolites from a marine-derived Aspergillus terreus B12. Arch Microbiol 203, 5621–5633 (2021). https://doi.org/10.1007/s00203-021-02548-4

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  • DOI: https://doi.org/10.1007/s00203-021-02548-4

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