Evolvulus alsinoides, belonging to the family Convolvulaceae, is an important medicinal plant widely used as a nootropic in the Indian traditional medicine system. In the genus Evolvulus, no research on the chloroplast genome has been published. Hence, the present study focuses on annotation, characterization, identification of mutational hotspots, and phylogenetic analysis in the complete chloroplast genome (cp) of E. alsinoides. Genome comparison and evolutionary dynamics were performed with the species of Solanales. The cp genome has 114 genes (80 protein-coding genes, 30 transfer RNA, and 4 ribosomal RNA genes) that were unique with total genome size of 157,015 bp. The cp genome possesses 69 RNA editing sites and 44 simple sequence repeats (SSRs). Predicted SSRs were randomly selected and validated experimentally. Six divergent hotspots such as trnQ-UUG, trnF-GAA, psaI, clpP, ndhF, and ycf1 were discovered from the cp genome. These microsatellites and divergent hot spot sequences of the Taxa ‘Evolvulus’ could be employed as molecular markers for species identification and genetic divergence investigations. The LSC area was found to be more conserved than the SSC and IR region in genome comparison. The IR contraction and expansion studies show that nine genes rpl2, rpl23, ycf1, ycf2, ycf1, ndhF, ndhA, matK, and psbK were present in the IR-LSC and IR-SSC boundaries of the cp genome. Fifty-four protein-coding genes in the cp genome were under negative selection pressure, indicating that they were well conserved and were undergoing purifying selection. The phylogenetic analysis reveals that E. alsinoides is closely related to the genus Cressa with some divergence from the genus Ipomoea. This is the first time the chloroplast genome of the genus Evolvulus has been published. The findings of the present study and chloroplast genome data could be a valuable resource for future studies in population genetics, genetic diversity, and evolutionary relationship of the family Convolvulaceae.

Evolvulus alsinoides属于旋花科，是一种重要的药用植物，在印度传统医学体系中被广泛用作促智药。在Evolvulus 属中，尚未发表关于叶绿体基因组的研究。因此，本研究的重点是在E. alsinoides的完整叶绿体基因组 (cp) 中的注释、表征、突变热点的识别和系统发育分析。. 对茄目物种进行基因组比较和进化动力学。cp 基因组有 114 个基因（80 个蛋白质编码基因、30 个转移 RNA 和 4 个核糖体 RNA 基因），它们是独特的，总基因组大小为 157,015 bp。cp基因组拥有69个RNA编辑位点和44个简单序列重复（SSR）。预测的 SSR 是随机选择并通过实验验证的。从cp基因组中发现了6个不同的热点，如trnQ-UUG、trnF-GAA、psaI、clpP、ndhF和ycf1 。进化分类群的这些微卫星和不同的热点序列' 可以用作物种鉴定和遗传差异调查的分子标记。在基因组比较中发现 LSC 区域比 SSC 和 IR 区域更保守。IR 收缩和扩展研究表明 9 个基因rpl2、rpl23、ycf1、ycf2、ycf1、ndhF、ndhA、matK和psbK存在于 cp 基因组的 IR-LSC 和 IR-SSC 边界中。cp基因组中的54个蛋白质编码基因处于负选择压力下，表明它们具有良好的保守性并且正在进行纯化选择。系统发育分析表明，E. alsinoides与Cressa属密切相关，但与Ipomoea属有一些分歧。这是首次发表演化属的叶绿体基因组。本研究的结果和叶绿体基因组数据可能是未来研究旋花科种群遗传学、遗传多样性和进化关系的宝贵资源。