In this study, we successfully established a Bactrocera dorsalis (Diptera: Tephritidae) embryonic cell line, i.e., QAU-Bd-E-2, from the insect eggs. The cells have been stably passaged for more than 60 times in TNM-FH medium with 10% fetal bovine serum (FBS). QAU-Bd-E-2 cells are adherent cells. Most of the cells were round, spindle-shaped, and rod-shaped. Round cells accounted for 82.3%, with a diameter of 13.9 ± 2.6 µm; spindle-shaped cells accounted for 9.8%, with the size of 51.2 ± 11.2 µm × 10.3 ± 3.1 µm; the rod-shaped cells accounted for 7.9%, with the size of 35.2 ± 9.4 µm × 12.0 ± 2.5 µm. The mitochondrial cytochrome oxidase I subunit (CoI) gene from QAU-Bd-E-2 cells was amplified, and the 657 bp fragment had a 100% similarity with the CoI gene of B. dorsalis, suggesting that the cell line was derived from B. dorsalis. The chromosome number of QAU-Bd-E-2 cells was mostly 12, which is the same as the B. dorsalis chromosome number. The cell density of QAU-Bd-E-2 cells reached the maximum (3.4 × 10⁶ cells/mL) at 192 h, and the population doubling time was 31.9 h. Bactrocera dorsalis cripavirus (BdCV) could replicate in QAU-Bd-E-2 cells, suggesting that this cell line could be used for in-depth study of the relationship between virus and host.

在这项研究中，我们成功地从昆虫卵中建立了一个背实蝇（双翅目：Tephritidae）胚胎细胞系，即QAU-Bd-E-2。细胞已在含 10% 胎牛血清 (FBS) 的 TNM-FH 培养基中稳定传代 60 次以上。QAU-Bd-E-2 细胞是贴壁细胞。大多数细胞呈圆形、纺锤形和棒状。圆形细胞占82.3%，直径为13.9±2.6 µm；梭形细胞占9.8%，大小为51.2 ± 11.2 µm × 10.3 ± 3.1 µm；棒状细胞占 7.9%，大小为 35.2 ± 9.4 µm × 12.0 ± 2.5 µm。扩增QAU-Bd-E-2细胞线粒体细胞色素氧化酶I亚基(CoI)基因，657 bp片段与B. dorsalis CoI基因100%相似，表明该细胞系来源于B. dorsalis。QAU-Bd-E-2细胞染色体数目多为12条，与B. dorsalis染色体数目相同。QAU-Bd-E-2细胞的细胞密度在192 h时达到最大值（3.4×10 ^{6 个}细胞/mL），群体倍增时间为31.9 h。Bactrocera dorsalis cripavirus (BdCV) 可以在 QAU-Bd-E-2 细胞中复制，表明该细胞系可用于深入研究病毒与宿主之间的关系。