Soil enzymes quickly respond to the disturbances caused in environmental pollution originating from human activities. Computation of enzyme activities has been used as indexes of microbial functionality, soil fertilization, biochemical cycling of numerous components in soil, extent of contamination, and ecosystem succession. Soil enzyme activity is one of the soil biological properties used as an indicator of soil quality due to their interrelationship to soil biology, being sensitive, integrative, ease to measure, “biological fingerprints” of previous soil management, indicator of biological symmetry, fertility, and changes in biological status of soil due to pollution. Dehydrogenase (DHG) enzymes are one of the most important components of soil enzymatic assay, as they determine the correct order of all biochemical pathways in soil biogeochemical cycles. While dehydrogenase activity (DHA) is calculated utilizing the procedures INT and TTC substrate in soil at the same time, a number of authors expressed their views about the unsatisfactory results when TTC has applied as substrate. Most of the researchers have applied incubation periods of 24 h at 37 °C, but some are modified it to 6 h 37 °C with either glucose or yeast extract as electron-donating substrate. Generally, in coal mine spoil, DHG functionality seems to lower, could be owing to the damage microflora, lack of soil organic matter. Measurement of DHA is frequently used as an extent of any disturbance owing to fertilizers, heavy metals, or other soil amendment (for management) practices, or, instantaneously degree of microbial functionality of soil.

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中文翻译：

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影响矿山退化土地中脱氢酶活性的不同土壤因素——最新技术回顾

土壤酶对人类活动造成的环境污染造成的干扰做出快速反应。酶活性的计算已被用作微生物功能、土壤施肥、土壤中多种成分的生化循环、污染程度和生态系统演替的指标。土壤酶活性是用作土壤质量指标的土壤生物学特性之一，因为它们与土壤生物学的相互关系敏感、综合、易于测量、以前土壤管理的“生物指纹”、生物对称性、肥力、以及污染引起的土壤生物状态的变化。脱氢酶 (DHG) 酶是土壤酶学测定中最重要的成分之一，因为它们确定了土壤生物地球化学循环中所有生化途径的正确顺序。虽然脱氢酶活性 (DHA) 是同时利用土壤中的 INT 和 TTC 底物程序计算的，但许多作者表达了他们对将 TTC 用作底物时结果不令人满意的看法。大多数研究人员在 37 °C 下应用了 24 小时的潜伏期，但有些研究人员将其修改为 6 小时 37 °C，并使用葡萄糖或酵母提取物作为给电子底物。一般来说，在煤矿弃土中，DHG 功能似乎较低，可能是由于微生物群落受到破坏，土壤有机质缺乏。DHA 的测量经常被用作肥料、重金属或其他土壤改良剂（用于管理）做法引起的任何干扰的程度，或者，

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