The present study deals with the synthesis of selenium nanoparticles (SeNPs) using Nilgirianthus ciliatus leaf extracts, characterized by UV-Vis spectrophotometer, XRD, FTIR, FE-SEM, HR-TEM, DLS, and zeta potential analysis. The antimicrobial activity against Staphylococcus aureus (MTCC96), Escherichia coli (MTCC443), and Salmonella typhi (MTCC98) showed the remarkable inhibitory effect at 25 µl/mL concentration level. Furthermore, the characterized SeNPs showed a great insecticidal activity against Aedes aegypti in the early larval stages with the median Lethal Concentration (LC50) of 0.92 mg/L. Histopathological observations of the SeNPs treated midgut and caeca regions of Ae. aegypti 4th instar larvae showed damaged epithelial layer and fragmented peritrophic membrane. In order to provide a mechanistic approach for further studies, molecular docking studies using Auto Dock Vina were performed with compounds of N. ciliatus within the active site of AeSCP2. Overall, the N. ciliates leaf-mediated biogenic SeNPs was promisingly evidenced to have potential larvicidal and food pathogenic bactericidal activity in an eco-friendly approach.

中文翻译：

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Nilgirianthus ciliates 叶提取物介导的硒纳米颗粒的绿色合成对食源性病原微生物的抗菌活性和对埃及伊蚊的分子对接的杀虫活性。

本研究涉及使用 Nilgirianthus ciliatus 叶提取物合成硒纳米颗粒 (SeNPs)，通过紫外-可见分光光度计、XRD、FTIR、FE-SEM、HR-TEM、DLS 和 zeta 电位分析进行表征。对金黄色葡萄球菌 (MTCC96)、大肠杆菌 (MTCC443) 和伤寒沙门氏菌 (MTCC98) 的抗菌活性在 25 µl/mL 浓度水平下显示出显着的抑制作用。此外，所表征的SeNPs在早期幼虫阶段表现出对埃及伊蚊的强大杀虫活性，中位致死浓度（LC50）为0.92 mg / L。SeNPs 处理的 Ae 的中肠和盲肠区域的组织病理学观察。埃及伊蚊第 4 龄幼虫上皮层受损，围营养膜破碎。为了提供进一步研究的机制方法，使用 Auto Dock Vina 的分子对接研究是在 AeSCP2 的活性位点内使用纤毛猪笼草的化合物进行的。总体而言，N. ciliates 叶介导的生物源 SeNPs 有希望地被证明具有潜在的杀幼虫和食品致病性杀菌活性。