Background

Helicobacter pylori infection and heterogeneity in its pathogenesis could describe diversity in the expression of inflammatory genes in the gastric tissue. We aimed to investigate transcriptional alteration of genes linked to gastritis concerning the H. pylori infection status and its virulence factors.

Methods and results

Biopsy samples of 12 infected and 12 non-infected patients with H. pylori that showed moderate chronic gastritis were selected for transcriptional analysis. Genotyping of H. pylori strains was done using PCR and relative expression of inflammatory genes was compared between the infected and non-infected patients using relative quantitative real-time PCR. Positive correlations between transcriptional changes of IL8 with TNF-α and Noxo1 in the infected and TNF-α with Noxo1, MMP7, and Atp4A in the non-infected patients were detected. Six distinct genotypes of H. pylori were detected that showed no correlation with gender, ethnicity, age, endoscopic findings, and transcriptional levels of host genes. Irrespective of the characterized genotypes, our results showed overexpression of TNF-α, MMP7, Noxo1, and ATP4A in the infected and IL-8, Noxo1, and ATP4A in the non-infected patients.

Conclusions

A complexity in transcription of genes respective to the characterized H. pylori genotypes in the infected patients was detected in our study. The observed difference in co-regulation of genes linked to gastritis in the infected and non-infected patients proposed involvement of different regulatory pathways in the inflammation of the gastric tissue in the studied groups.

中文翻译：

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与幽门螺杆菌感染状态及其毒力因子相关的胃炎基因的转录改变

背景

幽门螺杆菌感染及其发病机制的异质性可以描述胃组织中炎症基因表达的多样性。我们旨在研究与胃炎相关的基因的转录改变，涉及幽门螺杆菌感染状态及其毒力因子。

方法和结果

选择显示中度慢性胃炎的 12 名感染和 12 名未感染的幽门螺杆菌患者的活检样本进行转录分析。使用 PCR 对H. pylori菌株进行基因分型，并使用相对定量实时 PCR 比较感染和未感染患者之间炎症基因的相对表达。检测到感染患者中IL8与TNF-α和Noxo1的转录变化以及未感染患者中TNF-α与Noxo1、MMP7和Atp4A的转录变化之间的正相关。六种不同的幽门螺杆菌基因型检测到与性别、种族、年龄、内窥镜检查结果和宿主基因转录水平无关。不考虑特征基因型，我们的结果显示感染患者中的TNF-α、MMP7、Noxo1和ATP4A过表达，而未感染患者中的IL-8、Noxo1和ATP4A过表达。

结论

在我们的研究中检测到与感染患者的特征性幽门螺杆菌基因型相关的基因转录的复杂性。在感染和未感染患者中观察到的与胃炎相关的基因共同调节的差异表明，不同的调节途径参与了研究组中胃组织的炎症。