Symbiotic Paraburkholderia have been increasingly studied in the past 20 years, especially when associated with Mimosa; however, studies with native/endemic species are still scarce. In this study, thirty strains were isolated from root nodules of native Mimosa paranapiacabae and M. micropteris in two locations of the Campos Gerais. The BOX-PCR fingerprinting revealed high genomic diversity, and the 16S rRNA phylogeny clustered the strains in three distinct groups (GI, GII, GIII), with one strain occupying an isolated position. Phylogenetic analysis with four concatenated housekeeping genes (atpD + gltB + gyrB + recA) confirmed the same clusters of 16S rRNA, and the closest species were P. nodosa BR 3437T and P. guartelaensis CNPSo 3008T; this last one isolated from another Mimosa species of the Campos Gerais. The phylogenies of the symbiotic genes nodAC and nifH placed all strains in a well-supported branch with the other species of the symbiovar mimosae. The phylogenetic analyses indicated that the strains represent novel lineages of sv. mimosae and that endemic Mimosa coevolved with indigenous Paraburkholderia in their natural environments.

中文翻译：

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巴西保护区根瘤菌结瘤原生含羞草的分子多样性。

在过去的 20 年里，越来越多的人对共生的 Paraburkholderia 进行了研究，尤其是与含羞草相关时；然而，对本地/特有物种的研究仍然很少。在这项研究中，从吉拉斯州两个地点的本地含羞草 paranapiacabae 和 M. micropteris 的根瘤中分离出 30 个菌株。BOX-PCR 指纹图谱揭示了高基因组多样性，16S rRNA 系统发育将菌株聚集在三个不同的组（GI、GII、GIII）中，其中一个菌株占据一个孤立的位置。四个串联管家基因（atpD + gltB + gyrB + recA）的系统发育分析证实了相同的 16S rRNA 簇，最接近的物种是 P. nodosa BR 3437T 和 P. guartelaensis CNPSo 3008T；这最后一个从 Campos Gerais 的另一种含羞草中分离出来。共生基因 nodAC 和 nifH 的系统发育将所有菌株与含羞草共生体的其他物种置于一个得到良好支持的分支中。系统发育分析表明菌株代表 sv 的新谱系。含羞草和当地特有的含羞草在自然环境中与土著 Paraburkholderia 共同进化。