Abstract
Symbiotic Paraburkholderia have been increasingly studied in the past 20 years, especially when associated with Mimosa; however, studies with native/endemic species are still scarce. In this study, thirty strains were isolated from root nodules of native Mimosa paranapiacabae and M. micropteris in two locations of the Campos Gerais. The BOX-PCR fingerprinting revealed high genomic diversity, and the 16S rRNA phylogeny clustered the strains in three distinct groups (GI, GII, GIII), with one strain occupying an isolated position. Phylogenetic analysis with four concatenated housekeeping genes (atpD + gltB + gyrB + recA) confirmed the same clusters of 16S rRNA, and the closest species were P. nodosa BR 3437T and P. guartelaensis CNPSo 3008T; this last one isolated from another Mimosa species of the Campos Gerais. The phylogenies of the symbiotic genes nodAC and nifH placed all strains in a well-supported branch with the other species of the symbiovar mimosae. The phylogenetic analyses indicated that the strains represent novel lineages of sv. mimosae and that endemic Mimosa coevolved with indigenous Paraburkholderia in their natural environments.
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Data availability
The following new sequences have been deposited in GenBank/EMBL/DDBJ: 16S rRNA of CNPSo 3268 (MK839064), CNPSo 3270 (MK839065), CNPSo 3272 (MK839066), CNPSo 3273 (MK839067), CNPSo 3274 (MK839068), CNPSo 3276 (MK839069), CNPSo 3278 (MK839070), CNPSo 3281 (MK839071), CNPSo 3031 (KX809741), CNPSo 3057 (KX809740), CNPSo 3058 (KX809744), CNPSo 3076 (KX809742), CNPSo 3077 (KX809743); atpD of CNPSo 3268 (MT275606), CNPSo 3270 (MT275607), CNPSo 3272 (MT275608), CNPSo 3273 (MT275609), CNPSo 3274 (MT275610), CNPSo 3276 (MT275611), CNPSo 3278 (MT275612), CNPSo 3281 (MT275613), CNPSo 3031 (MT275601), CNPSo 3057 (MT275602), CNPSo 3058 (MT275603), CNPSo 3076 (MT275604), CNPSo 3077 (MT275605); gltB of CNPSo 3268 (MT275619), CNPSo 3270 (MT275620), CNPSo 3272 (MT275621), CNPSo 3273 (MT275622), CNPSo 3274 (MT275623), CNPSo 3276 (MT275624), CNPSo 3278 (MT275625), CNPSo 3281 (MT275626), CNPSo 3031 (MT275614), CNPSo 3057 (MT275615), CNPSo 3058 (MT275616), CNPSo 3076 (MT275617), CNPSo 3077 (MT275618); gyrB of CNPSo 3268 (MK844604), CNPSo 3270 (MK844605), CNPSo 3272 (MK844606), CNPSo 3273 (MK844607), CNPSo 3274 (MK844608), CNPSo 3276 (MK844609), CNPSo 3278 (MK844610), CNPSo 3281 (MK844611), CNPSo 3031 (MK844613), CNPSo 3057 (MK844612), CNPSo 3058 (MK844616), CNPSo 3076 (MK844614), CNPSo 3077 (MK844615); recA of CNPSo 3268 (MK844617), CNPSo 3270 (MK844618), CNPSo 3272 (MK844619), CNPSo 3273 (MK844620), CNPSo 3274 (MK844621), CNPSo 3276 (MK844622), CNPSo 3278 (MK844623), CNPSo 3281 (MK844624), CNPSo 3031 (MK844626), CNPSo 3057 (MK844625), CNPSo 3058 (MK844629), CNPSo 3076 (MK844627), CNPSo 3077 (MK844628); nifH of CNPSo 3268 (MT465896), CNPSo 3270 (MT465897), CNPSo 3272 (MT465898), CNPSo 3273 (MT465899), CNPSo 3276 (MT465900), CNPSo 3278 (MT465901), CNPSo 3057 (MT465892), CNPSo 3058 (MT465893), CNPSo 3076 (MT465894), CNPSo 3077 (MT465895); nodA of CNPSo 3268 (MT465911), CNPSo 3270 (MT465912), CNPSo 3272 (MT465913), CNPSo 3273 (MT465914), CNPSo 3276 (MT465915), CNPSo 3278 (MT465916), CNPSo 3057 (MT465917), CNPSo 3058 (MT465918), CNPSo 3076 (MT465919), CNPSo 3077 (MT465920); nodC of CNPSo 3268 (MK844631), CNPSo 3270 (MK844632), CNPSo 3272 (MK844633), CNPSo 3273 (MK844634), CNPSo 3274 (MK844635), CNPSo 3276 (MK844636), CNPSo 3278 (MK844637), CNPSo 3281 (MK844638), CNPSo 3031 (KY468306), CNPSo 3057 (MK844639), CNPSo 3058 (KY468307), CNPSo 3076 (MK844640), CNPSo 3077 (MK844641).
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Acknowledgements
M.S. Klepa and F. Paulitsch acknowledge Ph.D fellowships from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Finance Code 001). V.J.B.F. Nascimento acknowledges a research fellow from Fundação Araucária. M. Hungria acknowledges a research fellow from CNPq (Brazilian Council for Scientific and Technological Development).
Funding
This study was partially supported by INCT—Plant Growth Promoting Microorganisms for Agricultural Sustainability and Environmental Responsibility/Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 465133/2014-4), Fundação Araucária-STI 043/2019, CAPES and Fundação Araucária (09/2016).
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Klepa, M.S., Janoni, V., Paulitsch, F. et al. Molecular diversity of rhizobia-nodulating native Mimosa of Brazilian protected areas. Arch Microbiol 203, 5533–5545 (2021). https://doi.org/10.1007/s00203-021-02537-7
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DOI: https://doi.org/10.1007/s00203-021-02537-7